Western blot shows lysates of HEK293 human embryonic kidney cell line untreated (-) or treated (+) with 1 µg/mL insulin for 5 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-WNK1 (T60) Monoclonal Antibody (Catalog # MAB4720) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-WNK1 (T60) at approximately 280 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Phospho-WNK1 (T60) Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: HEK293 human embryonic kidney cell line treated with insulin

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: WNK1

With No Lysine (K) 1 (WNK1) belongs to a novel family of serine/threonine protein kinases involved in regulating ion transport. The WNKs contain a cysteine instead of the usual lysine in their kinase activation domains. WNK1 mutations have been implicated in Pseudohypoaldosteronism type II (PHAII), an autosomal dominant disorder characterized by hypertension, increased renal salt absorption, and impaired potassium and hydrogen excretion. WNK1 is phosphorylated by Akt1 and Akt2 at T60, and may function as a negative regulator of insulin-stimulated mitogenesis.

Long Name

WNK Lysine-deficient Protein Kinase 1

Alternate Names

KDP, PHA2C, PRKWNK1

Entrez Gene IDs

65125 (Human); 232341 (Mouse); 116477 (Rat)

Gene Symbol

WNK1

UniProt

Q9H4A3

Additional WNK1 Products

Product Documents for Human Phospho-WNK1 (T60) Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Phospho-WNK1 (T60) Antibody

For research use only

Citations
Reviews

Human Phospho-WNK1 (T60) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4720

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all WNK1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Phospho-WNK1 (T60) Antibody

Detection of Human Phospho-WNK1 (T60) by Western Blot.