Phosphopeptide containing human STAT5b Y699 site (amino acid sequence of this peptide is identical to a corresponding region of human STAT5a containing Y694).
Accession # P51692

Specificity

Detects human STAT5a/b when phosphorylated at Y694/Y699 in Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Scientific Data Images for Human Phospho-STAT5a/b (Y694/Y699) Antibody

Detection of Human Phospho-STAT5a/b (Y694/Y699) by Western Blot.

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN-aA (Catalog # 11100-1) for 20 minutes. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human Phospho-STAT5a/b (Y694/Y699) Monoclonal Antibody (Catalog # MAB4190) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-STAT5a/b (Y694/Y699) at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Phospho-STAT5a/b (Y694/Y699) in HeLa Human Cell Line.

STAT5a/b phosphorylated at Y694/Y699 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rat Anti-Human Phospho-STAT5a/b (Y694/Y699) Monoclonal Antibody (Catalog # MAB4190) at 0.3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Phospho-STAT5a/b (Y694/Y699) Antibody

Application

Recommended Usage

Immunocytochemistry

0.3-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Western Blot

2 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line treated with Recombinant Human IFN‑ alphaA (Catalog # 11100-1)

Reviewed Applications

Read 1 review rated 5 using MAB4190 in the following applications:

Immunocytochemistry/Immunofluorescence (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STAT5a/b

Signal Transduction and Activator of Transcription 5B (STAT5b) is a member of the Jak/STAT signal transduction pathway and is activated by a variety of cytokines (IL-22, IL-6, IFN alpha). Upon cytokine activation, STAT5b is phosphorylated and translocates to the nucleus where it binds to the DNA consensus site TTCN₃GAA and activates transcription of specific genes. STAT5b shares 93% amino acid identity with STAT5a. STAT5b is involved in a wide array of biological processes ranging from regulating apoptosis to adult mammary gland proliferation, differentiation and survival.

Long Name

Signal Transducer and Activator of Transcription 5b

Alternate Names

MGF, STAT5, STAT5A signal transducer and activator of transcription 5A

UniProt

P51692

Additional STAT5a/b Products

Product Documents for Human Phospho-STAT5a/b (Y694/Y699) Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Phospho-STAT5a/b (Y694/Y699) Antibody

For research use only

Citations
Reviews

Human Phospho-STAT5a/b (Y694/Y699) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4190

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications