Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 10 minutes or 2mM H₂O₂for 2 minutes. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Phospho-Lck (Y505) Monoclonal Antibody (Catalog # MAB7369) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-Lck (Y505) at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Phospho-Lck (Y505) in Jurkat Human Cell Line.

Lck phosphorylated at Y505 was detected in immersion fixed Jurkat human acute T cell leukemia cell line stimulated (upper panel) or not (lower panel) with pervanadate using Mouse Anti-Human Phospho-Lck (Y505) Monoclonal Antibody (Catalog # MAB7369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human Phospho-Lck (Y505) Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed Jurkat human acute T cell leukemia cell line stimulated with pervanadate

Western Blot

0.5 µg/mL
Sample: Jurkat human acute T cell leukemia cell line treated with Pervanadate or H₂O₂

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lck

Lck (p56lck) is a 56 kDa cytosolic phosphoprotein within the Src family of non-receptor tyrosine kinases. The 509 amino acid (aa) human Lck contains an SH3 domain (aa 61-121), an SH2 domain (aa 127-224) and a protein kinase domain (aa 251-259). Within aa 2-66, human Lck shares 89% aa sequence identity with mouse and rat Lck. A short (363 aa) isoform contains alternate sequence starting at aa 348, while a 539 aa isoform contains inserted sequence after aa 321. Lck interacts with T cell CD4 and CD8 molecules and plays a pivotal role in regulating T cell activation. It can be overexpressed in cancers and functions as an oncogene.

Long Name

Lymphocyte Protein Tyrosine Kinase

Alternate Names

Hck-3, Lcktkr, LSTRA, p56lck, pp58lck, YT16

Entrez Gene IDs

3932 (Human); 16818 (Mouse); 313050 (Rat)

Gene Symbol

LCK

UniProt

P06239

Additional Lck Products

Product Documents for Human Phospho-Lck (Y505) Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human Phospho-Lck (Y505) Antibody

For research use only

Citations
Reviews

Human Phospho-Lck (Y505) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7369

Key Product Details

Validated by

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Phospho-Lck (Y505) Antibody

Detection of Human Phospho-Lck (Y505) by Western Blot.