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Human Phospho-Insulin R (Y1162/Y1163)/IGF-I R (Y1135/Y1136) Antibody

Bio-Techne includes R&D Systems | Catalog # AF2507

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AF2507-SP
AF2507

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

CyTOF-ready, Immunocytochemistry, Intracellular Staining by Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Product Summary

Immunogen

Phosphopeptide containing human Insulin R Y1162/Y1163 sites

Specificity

Detects human Insulin R dually phosphorylated at Y1162 and Y1163, and human IGF-I R dually phosphorylated at Y1135 and Y1136.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Phospho-Insulin R (Y1162/Y1163)/IGF-I R (Y1135/Y1136) Antibody

Detection of Human Phospho-Insulin R (Y1162/Y1163) and Phospho-IGF-I R (Y1135/1136) antibody by Western Blot.

Detection of Human Phospho‑Insulin R (Y1162/Y1163) and Phospho‑IGF‑I R (Y1135/1136) by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 100 µM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-Insulin R (Y1162/Y1163)/IGF-I R (Y1135/Y1136) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2507), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-Insulin R (Y1162/Y1163) at 95 kDa and Phospho-IGF-I R (Y1135/1136) at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Phospho-Insulin R (Y1162/1163)/IGF-I R (Y1135/1136) antibody in A431 Human Cell Line by Immunocytochemistry (ICC).

Phospho‑Insulin R (Y1162/1163)/IGF-I R (Y1135/1136) in A431 Human Cell Line.

Insulin R phosphorylated at Y1162/1163 and IGF-I R phsophorylated at Y1135/1136 were detected in immersion fixed A431 human epithelial carcinoma cell line untreated (lower panel) or treated (upper panel) with pervanadate using Rabbit Anti-Human Phospho-Insulin R (Y1162/1163)/IGF-I R (Y1135/1136) Cross-reactive Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2507) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Insulin R/CD220 antibody in HeLa Human Cell Line antibody by Flow Cytometry.

Detection of Insulin R/CD220 in HeLa Human Cell Line by Flow Cytometry.

HeLa human cervical epithelial carcinoma cell line was unstimulated (light orange open histogram) or treated with 100 µM pervanadate for 15 minutes, then stained with Rabbit Anti-Human Phospho-Insulin R (Y1162/1163)/IGF-I R (Y1135/1136) cross-reactive Anti-gen Affinity-purified Polyclonal Antibody (Catalog # AF2507, dark orange filled histogram) or isotype control antibody (Catalog # AB-105-C, blue open histogram), followed by Phy-coerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.

Applications for Human Phospho-Insulin R (Y1162/Y1163)/IGF-I R (Y1135/Y1136) Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed A431 human epithelial carcinoma cell line treated with pervanadate

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line treated with pervanadate, fixed with paraformaldehyde, and permeabilized with methanol

Western Blot

0.5 µg/mL
Sample: Pervanadate-treated A431 human epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 5 publications using AF2507 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Insulin R/IGF-I R Heterotetramer

The heterotetrameric receptors for insulin (INS R) and IGF-I (IGF-I R) are receptor tyrosine kinases that consist of two ligand-binding alpha subunits and two beta subunits. Ligand binding induces autophosphorylation on multiple tyrosine residues of beta subunits. Phosphorylation of Tyr1162 and 1163 on INS R and Tyr1135 and 1136 on IGF‑I R stimulates intrinsic kinase activity.

Long Name

Insulin Receptor

Additional Insulin R/IGF-I R Heterotetramer Products

Product Documents for Human Phospho-Insulin R (Y1162/Y1163)/IGF-I R (Y1135/Y1136) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Phospho-Insulin R (Y1162/Y1163)/IGF-I R (Y1135/Y1136) Antibody

For research use only

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