Western blot shows lysates of MDA-MB-453 human breast cancer cell line untreated (-) or treated (+) with 300 ng/mL Recombinant Human NRG1-beta /HRG1-beta (Catalog # 396-HB) for 5 minutes. PVDF membrane was probed with 1 µg/mL of Human Phospho-ErbB3/Her3 (Y1262) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5817), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-ErbB3/Her3 (Y1262) at approximately 185 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Phospho-ErbB3/Her3 (Y1262) by Western Blot

NRG1-induced proliferation and signaling of Ba/F3 cells expressing ERBB3 and/or ERBB4.(A) Cells expressing either ERBB4 (upper panel), ERBB4 and ERBB3 (middle panel) or ERBB3 receptors (lower panel) were cultured in medium without ligand, in the presence of 10 ng/ml IL3, or in the presence of 10 or 100 ng/ml NRG1. Cell numbers were counted at the indicated time points. (B) Parental Ba/F3 or Ba/F3 cells expressing ERBB3, ERBB4 or both receptors were incubated in the absence of IL3 and treated with 100 ng/mL NRG1 where indicated. Total cell lysate were then prepared and immunoblotted with phosphoepitope- and protein-specific antibodies for ERBB3, ERBB4, Akt and MEK. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26745281), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Phospho-ErbB3/Her3 (Y1262) Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: MDA-MB-453 human breast cancer cell line treated with Recombinant Human NRG1-beta /HRG1-beta (Catalog # 396-HB)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ErbB3/Her3

ErbB3 (also known as HER3) is a type I transmembrane growth factor receptor and member of the human epidermal growth factor receptor (HER) family. ErbB3 is unique among HER family members in that it contains a defective kinase domain, and is therefore active only as a partner in a heterodimer complex with other ErbBs. Despite ErbB3's lack of intrinsic kinase activity, these heterodimers can be quite potent: the ErbB2/ErbB3 complex is considered the most active ErbB signaling dimer for the activation of downstream pathways. In addition, monomeric ErbB3 serves as a low affinity receptor for the heregulins (HRG), while ErbB2/ErbB3 heterodimers form a high affinity receptor complex. Phosphorylation of ErbB3 at Y1262 following ligand binding generates a binding site for the Grb2 adaptor protein.

Long Name

Receptor Tyrosine Protein Kinase ErbB3

Alternate Names

HER3

Entrez Gene IDs

2065 (Human); 13867 (Mouse)

Gene Symbol

ERBB3

Additional ErbB3/Her3 Products

Product Documents for Human Phospho-ErbB3/Her3 (Y1262) Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Phospho-ErbB3/Her3 (Y1262) Antibody

For research use only

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Human Phospho-ErbB3/Her3 (Y1262) Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5817

Key Product Details

Validated by

Species Reactivity

Validated:

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Validated:

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Label

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Product Specifications

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Specificity

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Host

Isotype

Scientific Data Images for Human Phospho-ErbB3/Her3 (Y1262) Antibody

Detection of Human Phospho-ErbB3/Her3 (Y1262) by Western Blot.