Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HEK293 human embryonic kidney cell line untreated (-) or treated (+) with 20 mJ/cm²ultraviolet light (UV) with a 30 minute recovery. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Phospho-CREB (S133) Monoclonal Antibody (Catalog # MAB6906) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-CREB (S133) at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Phospho-CREB (S133) in HeLa Human Cell Line.

CREB phosphorylated at A133 was detected in immersion fixed Hela human cervical epithelial carcinoma cells, unstimulated (lower panel) and stimulated (upper panel) with PMA, using Mouse Anti-Human Phospho-CREB (S133) Monoclonal Antibody (Catalog # MAB6906) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Phospho-CREB (S133) Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed Hela human cervical epithelial carcinoma cells stimulated with PMA

Western Blot

0.5 µg/mL
Sample: Hela human cervical epithelial carcinoma cells and HEK293 human embryonic kidney cells stimulated with ultraviolet light (UV).

Reviewed Applications

Read 4 reviews rated 4 using MAB6906 in the following applications:

Immunocytochemistry/Immunofluorescence (2 Reviews)
Western Blot (2 Reviews)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CREB

The cAMP response element binding protein (CREB) belongs to the bZIP superfamily of transcription factors, containing a basic domain that mediates DNA binding and a leucine zipper domain that facilitates dimerization. Within the promoter of target genes, CREB dimers bind cAMP response elements, defined by the palindromic consensus sequence TGACGTCA. When phosphorylated at Ser133, CREB also binds the coactivator CREB binding protein (CBP), which enhances transcription by acetylating histones to facilitate chromatin unraveling.

Long Name

cAMP Response Element-binding Protein

Alternate Names

CREB1

Entrez Gene IDs

1385 (Human); 12912 (Mouse)

Gene Symbol

CREB1

Additional CREB Products

Product Documents for Human Phospho-CREB (S133) Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Phospho-CREB (S133) Antibody

For research use only

Citations
Reviews

Human Phospho-CREB (S133) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6906

Key Product Details

Validated by

Species Reactivity

Applications

Label

Antibody Source

View all CREB Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Phospho-CREB (S133) Antibody

Detection of Human Phospho-CREB (S133) by Western Blot.