Human Perilipin-4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11760

R&D Systems, part of Bio-Techne
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MAB11760-SP
MAB11760-100
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1114804

View all Perilipin-4 Antibodies »

Product Specifications

Immunogen

Synthetic Peptide
Accession # Q96Q06

Specificity

Detects a synthetic peptide specific for human PLIN-4 around amino acid 1085 in Direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Perilipin-4 Antibody

Detection of Perilipin-4 in Human Breast.

Perilipin-4 was detected in immersion fixed paraffin-embedded sections of human breast using Mouse Anti-Human Perilipin-4 Monoclonal Antibody (Catalog # MAB11760) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog&(VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to adipose. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Perilipin-4 Antibody

Application
Recommended Usage

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Perilipin-4

Perilipin-4 (PLIN4) is a member of the perilipin protein family, with a molecular weight of approximately 135 kDa. The perilipin family comprises lipid droplet-associated proteins that play key roles in lipid metabolism, storage, and mobilization within cells. PLIN4 is primarily expressed in adipose tissue and other lipid-storing cells, where it is localized to the surface of lipid droplets, protecting them from untimely lipolysis and promoting efficient lipid storage. It is thought to act in conjunction with other members of the perilipin family to regulate lipid droplet dynamics and lipid release in response to metabolic cues. PLIN4 is implicated in diverse biological processes, including energy homeostasis and thermogenesis, and alterations in its expression have been associated with metabolic disorders such as obesity, diabetes, and fatty liver disease. Additionally, dysregulation of PLIN4 has been linked to lipid accumulation in tissues, emphasizing its critical role in lipid metabolism and energy storage. Recent research suggests a growing potential for PLIN4 as a biomarker for metabolic disease and as a target for therapeutic interventions aimed at modulating lipid storage and metabolic health.

References

  1. Brasaemle DL, Dolios G, Shapiro L, Wang R. Proteomic analysis of proteins associated with lipid droplets of basal and lipolytically stimulated 3T3-L1 adipocytes. J Biol Chem. 2004 Nov 5;279(45):46835-42. doi: 10.1074/jbc.M409340200. Epub 2004 Aug 27. PMID: 15337753.
  2. Kimmel AR, Sztalryd C. The Perilipins: Major Cytosolic Lipid Droplet-Associated Proteins and Their Roles in Cellular Lipid Storage, Mobilization, and Systemic Homeostasis. Annu Rev Nutr. 2016 Jul 17;36:471-509. doi: 10.1146/annurev-nutr-071813-105410. PMID: 27431369.
  3. Sztalryd C, Brasaemle DL. The perilipin family of lipid droplet proteins: Gatekeepers of intracellular lipolysis. Biochim Biophys Acta Mol Cell Biol Lipids. 2017 Oct;1862(10 Pt B):1221-1232. doi: 10.1016/j.bbalip.2017.07.009. Epub 2017 Jul 25. PMID: 28754637; PMCID: PMC5595658.

Alternate Names

KIAA1881, MDRV, MRUPAV, NIPA2, S3-12

Entrez Gene IDs

729359 (Human); 57435 (Mouse); 363331 (Rat)

Gene Symbol

PLIN4

UniProt

Q96Q06 (Human)

Additional Perilipin-4 Products

Product Documents for Human Perilipin-4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Perilipin-4 Antibody

For research use only

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