Detects human PDGF-C in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant mouse PDGF-C is observed, and less than 1% cross-reactivity with human PDGF, recombinant human (rh) PDGF‑AA, rhPDGF‑BB, rhPDGF‑AB, and rhPDGF‑D is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human PDGF-C Antibody

Detection of Human PDGF‑C by Western Blot.

Western blot shows lysates of human platelets. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PDGF-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1560) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for PDGF-C p80 at approximately 80 kDa and PDGF-C p55 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

PDGF-C in Human Pancreatic Cancer Tissue.

PDGF-C was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using 1.7 µg/mL Goat Anti-Human PDGF-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1560) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Cell Proliferation Induced by PDGF-CC and Neutralization by Human PDGF-C Antibody.

Recombinant Human PDGF-CC (Catalog # 1687-CC) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF-CC (0.8 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human PDGF-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1560). The ND₅₀ is typically 6-24 µg/mL.

Detection of Human PDGF-C by Western Blot

PDGF-C binds to NRP-1 in vitro and stimulates signal transduction in M14-N cells(A) PDGF-C binding to NRP-1 was assayed in Maxisorp Nunc immunoplates coated with the growth factor and incubated with a solution containing 1 μg/ml NRP-1/hFc chimera. Bound NRP-1/hFc chimeric polypeptide was quantified using an alkaline phosphatase-conjugated anti-human Fc antibody. PDGFR alpha/hFc and VEGFR-2/hFc chimeras were used as positive and negative controls, respectively. Each value represents the mean of three independent determinations (± SD). ANOVA followed by Bonferroni’s post-hoc test: p<0.05 (*). (B) The effect of pre-incubation of selected PDGF-C-coated wells with graded concentrations of goat anti-PDGF-C or IgG control antibodies on PDGF-C binding to NRP-1 was evaluated using the binding assay described in panel A. Student’s t-test: p<0.05 (*), p<0.01 (**). (C) M14-N cells were untreated or treated with 50 ng/ml PDGF-C for the indicated times and p130Cas phosphorylation levels were evaluated by Western blot. Total p130Cas was detected to determine the relative amount of phosphorylated protein and beta-tubulin expression was tested as loading control. Protein extracts from M14-C cells, at time 5 min, were included in the analysis as negative control. A representative experiment out of three is shown. (D) Densitometric quantification of the levels of phospho-p130Cas relative to the total amount of p130Cas, after normalization by beta-tubulin content in the samples. Histogram represents the mean values (± SD) of three independent determinations. Student’s t-test analysis: p<0.01 (**). NS, non-stimulated cells. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28977999), licensed under a CC-BY license. Not internally tested by R&D Systems.

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Applications for Human PDGF-C Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreatic cancer tissue

Western Blot

1 µg/mL
Sample: Human platelets

Neutralization

Measured by its ability to neutralize PDGF‑CC-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line [Raines, E.W. et al. (1985) Methods Enzymol. 109:749]. The Neutralization Dose (ND₅₀) is typically 6-24 µg/mL in the presence of 0.8 µg/mL Recombinant Human PDGF‑CC.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF-C

Platelet-derived growth factor C (PDGF-C), also named spinal cord derived growth factor (SCDGF) and follotain, is a member of the PDGF family that binds to the PDGF receptor alpha alpha and alpha beta. PDGF-C is a growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. It is a potent mitogen and chemoattractant for cells of mesenchymal origin. It is also required for normal skeleton formation during embryonic development, especially for normal development of the craniofacial skeleton and for normal development of the palate. In addition, PDGF-C is required for normal skin morphogenesis during embryonic development. PDGF-C plays an important role in angiogenesis and blood vessel development and is involved in fibrotic processes, in which transformation of interstitial fibroblasts into myofibroblasts plus collagen deposition occurs. The CUB domain has mitogenic activity in coronary artery smooth muscle cells, suggesting a role beyond the maintainance of the latency of the PDGF domain. In the nucleus, PDGFC seems to have additional function. Western blot analysis of platelets identified 55 kDa and 80 kDa PDGF C forms that were secreted on platelet activation (1).

References

Fang L. et al. (2004) Arterioscler Thromb Vasc Biol. 24:787

Long Name

Platelet-derived Growth Factor C

Alternate Names

PDGFC

Entrez Gene IDs

56034 (Human); 54635 (Mouse)

Gene Symbol

PDGFC

UniProt

Q9NRA1

Additional PDGF-C Products

Product Documents for Human PDGF-C Antibody

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Product Specific Notices for Human PDGF-C Antibody

For research use only

Citations
Reviews

Human PDGF-C Antibody

R&D Systems, part of Bio-Techne | Catalog # AF1560

Key Product Details

Species Reactivity

Validated:

Cited:

Applications