Human PDGF-BB Antibody

Name: Human PDGF-BB Antibody
Brand: R&D Systems
SKU: AB-220-NA

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AB-220-NA

Citations (4)

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Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human PDGF‑BB Ser82-Thr190 Accession # P01127
Applications:	Block/Neutralize, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human PDGF-BB in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant human (rh) PDGF-AB is observed, approximately 50% cross-reactivity with recombinant rat PDGF-B and natural porcine PDGF is observed, and less than 10% cross-reactivity with rhPDGF-A is observed.

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Applications for Human PDGF-BB Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human PDGF-BB (Catalog # 220-BB)

Item	Value
Neutralization	Measured by its ability to neutralize PDGF‑BB-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Raines, E. W. et al. (1985) Methods Enzymol. 109:749. The Neutralization Dose (ND₅₀) is typically 0.25-1.5 µg/mL in the presence of 20 ng/mL Recombinant Human PDGF‑BB.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human PDGF-BB Antibody

	Neutralization Cell Proliferation Induced by PDGF‑BB and Neutralization by Human PDGF‑BB Antibody. Recombinant Human PDGF-BB (Catalog # 220-BB) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human PDGF-BB (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human PDGF-BB Polyclonal Antibody (Catalog # AB-220-NA). The ND₅₀ is typically 0.25-1.5 µg/mL.

Formulation, Preparation and Storage

Purification	Protein A or G purified
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 1 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: PDGF-BB

Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha ‑granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

Item	Value
Long Name	Platelet-derived Growth Factor BB
Entrez Gene IDs	5155 (Human); 24628 (Rat)
Alternate Names	PDGF-BB, PDGFBB

Citations for Human PDGF-BB Antibody (4)

Showing 1 - 4 of 4 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human PDGF-BB Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Neutralization

SF Mause et al. (2022), Engagement of the CXCL12-CXCR4 Axis in the Interaction of Endothelial Progenitor Cell and Smooth Muscle Cell to Promote Phenotype Control and Guard Vascular Homeostasis International Journal of Molecular Sciences, 23(2).
PMID: 35055054
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

YY Liang et al. (2020), RASSF1A inhibits PDGFB-driven malignant phenotypes of nasopharyngeal carcinoma cells in a YAP1-dependent manner Cell Death Dis, 11(10):855.
PMID: 33057010
Species: Human
Sample Types: Synovial Fluid
Applications: Neutralization

Y Zou et al. (2018), Long noncoding RNA LERFS negatively regulates rheumatoid synovial aggression and proliferation J. Clin. Invest., 0(0).
PMID: 30198906
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Chanakira A et al. (2015), Hypoxia Differentially Regulates Arterial and Venous Smooth Muscle Cell Migration. PLoS ONE, 10(9):e0138587.
PMID: 26381529

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Showing 1 - 4 of 4 citations Showing All

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Human PDGF-BB Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human PDGF-BB Antibody

Scientific Data Examples for Human PDGF-BB Antibody

Neutralization

Formulation, Preparation and Storage

Reconstitution Calculator

Background: PDGF-BB

Citations for Human PDGF-BB Antibody (4)

Product Documents for Human PDGF-BB Antibody

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