Human PDCD4 Alexa Fluor® 350-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB7019U
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 808508
Product Specifications
Immunogen
E. coli-derived recombinant human PDCD4
Lys212-His358
Accession # Q53EL6
Lys212-His358
Accession # Q53EL6
Specificity
Detects human PDCD4 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human PDCD4 Alexa Fluor® 350-conjugated Antibody
Application
Recommended Usage
Immunocytochemistry
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: PDCD4
PDCD4 (Programmed cell death protein 4; also H731) is a 54-64 kDa member of the PDCD4 family of molecules. It is widely expressed, being found in mammary epithelium, CD34+ bone marrow progenitor cells, fibroblasts and keratinocytes. PDCD4 is both cytoplasmic and nuclear. In the cytoplasm, it blocks protein translation by binding to eIF4A, an act that dissociates eIF4G and mRNA from eIF4A. In the nucleus, it seems to block transcription of select genes, one of which is MAP4K1, a key enzyme in the AP-1-mediated transcription pathway. Human PDCD4 is 469 amino acids (aa) in length. It contains an NLS (aa 58-64), an MI domain (aa 163-284), another NLS (aa 241-250) and a second MI domain (aa 326-449). There are at least seven utilized Ser/Thr phosphorylation sites and one Tyr phosphorylation site. There are two potential splice forms, one that contains a deletion of aa 15-28, and a second that may show a three aa substitution for aa 2-15. Over aa 212-357, human PDCD4 shares 98% aa identity with mouse PDCD4.
Long Name
Programmed Cell Death 4
Alternate Names
H731, Protein 197/15a
Gene Symbol
PDCD4
UniProt
Additional PDCD4 Products
Product Specific Notices for Human PDCD4 Alexa Fluor® 350-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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