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Human PARD3/Par3 Alexa Fluor™ Plus 555-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB8030AFP555

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FAB8030AFP555-100UG

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Alexa Fluor Plus 555 (Excitation = 558 nm, Emission = 572 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 833517

Product Specifications

Immunogen

E. coli-derived recombinant human PARD3/Par3

Specificity

Detects human PARD3/Par3 in ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human PARD3b/Par3b is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PARD3/Par3

PARD3 (partitioning defective 3 homolog), also called Par3, is a widely expressed adapter protein involved in asymmetrical cell division, cell polarization, and tight junction formation. It forms a cell polarity complex with Par6 and aPKC (atypical protein kinase C), linking to PTEN (phosphatase and tensin homolog) in polarized epithelia and VE-Cadherin in polarized endothelia. The region used as an immunogen, human PARD3 amino acids (aa) 451-555 (of 1356 aa in full-length PARD3) shares 97% and 96% aa sequence identity with mouse and rat PARD3, respectively. It includes the second of three PDZ domains, a region which is involved in heterophilic interactions and is present within all 10 reported PARD3 isoforms that vary in length from 988 aa to 1356 aa.

Long Name

Par-3 Partitioning Defective 3 Homolog

Alternate Names

ASIP, Baz, Bazooka, PAR3, PAR3A, SE2-5L16, SE2-5LT1, SE2-5T2

Entrez Gene IDs

56288 (Human); 93742 (Mouse); 81918 (Rat)

Gene Symbol

PARD3

UniProt

Additional PARD3/Par3 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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