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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

E. coli-derived recombinant human PACT
Ser2-Lys313
Accession # O75569

Specificity

Detects human PACT in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human PACT Alexa Fluor® 700-conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PACT

PACT (PKR Activator; also HSD14, PRKRA and RAX [in mouse]) is a 34-37 kDa member of the PRKRA family of molecules. It is a ubiquitously expressed, cytoplasmic, 313 amino acid (aa) protein that contains three discrete double-stranded (ds) RNA binding motifs (aa 31-101; 126-194; 240-308). It exists as a monomer, homodimer, and heterodimer with at least two partners, TRBP and Dicer. Two isoform variants are known to exist, including one that utilizes an alternative start site at Met26, and another that shows a 10 aa substitution for aa 1-21. PACT is perhaps best known for its relationship with PKR, a 52-58 kDa Ser/Thr kinase that downmodulates protein synthesis and initiates apoptosis. PKR is constitutively silent, and activated via two principal ways; the first is through binding to (viral) dsRNA, and the second is through binding to an activator, PACT. PACT is kept in check upstream through dimerization with at 48-49 kDa regulatory protein termed TRBP. Following growth factor withdrawl or exposure to bacterial products such as LPS, PACT is phosphorylated at Ser287, and this inactive PACT:TRBP heterodimer dissociates, freeing up PACT to bind to PKR. PACT's interaction with PKR induces an autophosphorylation event on PKR. This allows for an activated PKR interaction with eIF2a, causing its phosphorylation and a subsequent reduction of protein synthesis. PACT may also act independently of PKR, and serve as a component of RISC, a complex that downregulates mRNA translation. Within this complex, PACT likely associates with Dicer in concert with Argonaute. Full-length PACT shares 98% aa sequence identity with the mouse ortholog to human PACT termed RAX.

Alternate Names

DYT16, HSD14, PKR-Associating Protein X, PRKRA, RAX

Entrez Gene IDs

8575 (Human); 23992 (Mouse); 311130 (Rat)

Gene Symbol

PRKRA

UniProt

Additional PACT Products

Product Documents for Human PACT Alexa Fluor® 700-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human PACT Alexa Fluor® 700-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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