Human P-Selectin/CD62P Antibody

Detection of P-Selectin/CD62P by Immunocytochemistry/ Immunofluorescence

KD platelets crosstalk with monocytes via forming “adhesion junctions”. A) Flow cytometry analysis showing CD62p expression in platelets from HS (n = 24) and KD patients (n = 24). Unpaired t test. B) Correlation between MPA and CD62p positive platelets in patients with acute KD (n = 24). p value was calculated using correlation analysis. C) Heatmap showing selected ligand–receptor interactions between MPA and subtypes of monocytes. D) Immunostaining of PSGL‐1 (blue), CD14 (green), CD41 (red), and CD62p (gray) in monocytes after coculture with KD platelets for 8 h. Activated CD41+ platelets (red) forming CD62p (gray) and PSGL‐1‐mediated junctions (blue) with CD14+ monocytes (green) are shown (n = 6). Scale bar: 5 µm. E) Immunostaining of CD11b (blue), CD14 (green), CD41 (red), GPIb alpha (gray) in monocytes after coculture with KD platelets for 8 h. Activated CD41+ platelets (red) forming GPIb alpha (gray) and CD11b‐mediated junctions (blue) with CD14+ monocytes (green) are shown (n = 6). Scale bar: 5 µm. F,G) Proximity ligation assay (PLA) showing adhesion junctions of CD62p–PSGL‐1 (F), GPIb alpha–CD11b (G) on monocytes. PLA staining: red; nuclei: blue; wheat germ agglutinin (WGA): green (n = 5). Scale bar: 10 µm. PLT, platelet; HS, healthy subject; KD, Kawasaki disease; MPA, platelet–monocyte aggregate; ITGAM, integrin subunit alpha M (CD11b). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39665236), licensed under a CC-BY license. Not internally tested by R&D Systems.