Human ORP150/HSP12A Alexa Fluor™ Plus 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5558AFP488
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ORP150/HSP12A
Specificity
Detects human ORP150/HSP12A in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) HSP-70, rhHSPA-2, and rhHSPH-1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: ORP150/HSP12A
ORP150 (Oxygen-related protein of 150 kDa) is a member of the heat shock protein 70 family of molecules. Although the predicted MW is 111 kDa, it runs at 150 kDa in SDS-PAGE. The discrepancy may not be anomalous, since it is secreted into the ER lumen and likely to be glycosylated and/or phosphorylated. ORP150 is highly expressed in cells involved in secretion such as pancreatic acinar cells and hepatocytes. Mature human ORP150 is 967 amino acids (aa) in length. It contains two HSP70 signatures (aa 230‑243 and 380‑394) and an ER-retention signal (aa 996‑999). There are multiple isoforms. Three show alternate start sites at Met88, Met152, and a Met 44 aa upstream of the standard start site. Four isoforms also show deletions of aa 148‑958, 51‑59, 603‑664 and 164‑507, respectively. There is one 44 aa substitution for aa 603‑999. Over aa 1‑994, human ORP150 shares 92% aa identity with mouse ORP150.
Long Name
Oxygen Regulated Protein, 150 kDa
Alternate Names
HSP12A, HYOU1
Gene Symbol
HYOU1
UniProt
Additional ORP150/HSP12A Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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