Human NRG1-beta 1/HRG1-beta 1 EGF Domain Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3771
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Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Rat
Applications
Validated:
ELISA Capture (Matched Antibody Pair)
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 147705
Product Specifications
Immunogen
E. coli-derived recombinant human NRG1-beta 1/HRG1-beta 1
Ser2-Lys246
Accession # AAA58639
Ser2-Lys246
Accession # AAA58639
Specificity
Detects human NRG1-beta 1 in ELISAs. Recognizes an epitope found in the EGF-like domain of NRG1‑ beta1 (amino acids 179‑246). It also detects recombinant human SMDF (Sensory and Motor neuron-Derived Factor), but it does not recognize the EGF-like domain of human NRG1-alpha (amino acids 177‑241). The epitope is present within amino acids 213‑230 of human NRG1-beta 1. This segment is present in all NRG1 beta isoforms, GGF‑2 (glial growth factor), and SMDF, but it is absent from NRG1-alpha, NDF43, and NRG1-gamma.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human NRG1-beta 1/HRG1-beta 1 EGF Domain Antibody
Human Neuregulin-1 beta 1 / NRG1 beta 1 ELISA Standard Curve
Recombinant Human NRG1-beta 1/HRG1-beta 1 Extracellular Domain (Catalog # 377-HB) was serially diluted and captured by Mouse Anti-Human NRG1-beta 1/HRG1-beta 1 EGF Domain Monoclonal Antibody (Catalog # MAB3771) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human NRG1-beta 1/HRG1-beta 1 Extracellular Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF377) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human NRG1-beta 1/HRG1-beta 1 EGF Domain Antibody
Human NRG1-beta 1/HRG1-beta 1 Sandwich Immunoassay
ELISA Capture (Matched Antibody Pair)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neuregulin-1 beta 1/NRG1 beta 1
Alternate Names
Neuregulin1 beta 1
Gene Symbol
NRG1
UniProt
Additional Neuregulin-1 beta 1/NRG1 beta 1 Products
Product Documents for Human NRG1-beta 1/HRG1-beta 1 EGF Domain Antibody
Product Specific Notices for Human NRG1-beta 1/HRG1-beta 1 EGF Domain Antibody
For research use only
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