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Human NPRA/NPR1 Antibody

Bio-Techne includes R&D Systems | Catalog # MAB48601

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MAB48601-100
MAB48601-SP

Key Product Details

Species Reactivity

Validated:

Human

Cited:

 Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 377018

Product Summary for Human NPRA/NPR1 Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human NPR1/NPRA
Gly33-Glu473
Accession # P16066

Specificity

Detects human NPR1/NPRA in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human NPRA/NPR1 Antibody

NPRA/NPR1 antibody in Human Kidney by Immunohistochemistry (IHC-P).

NPRA/NPR1 in Human Kidney.

NPRA/NPR1 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human NPRA/NPR1 Monoclonal Antibody (Catalog # MAB48601) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cell membranes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human NPRA/NPR1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 1 publication using MAB48601 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NPRA/NPR1

Natriuretic peptide receptor A/guanylate cyclase A (NPR1), also called NPRA or GC-A, is a 120‑140 kDa type I transmembrane glycoprotein that is the primary receptor for natrietic peptides ANP and BNP. Binding of ANP to the extracellular ligand binding domain (aa 54‑415), plus ATP to the intracellular kinase homology domain (aa 528‑805) activates a cytoplasmic guanylate cyclase (aa 840‑1023). NPR1 is expressed most highly in kidney, adrenal and adipose tissue. Human NPR1 extracellular domain shows 86%, 44% and 34% aa identity with mouse NPR1, human NPRB and human NPRC, respectively.

Long Name

Natriuretic Peptide Receptor A

Alternate Names

ANPa, ANPRA, GC-A, GUCY2A, NPR1, NPRA, Pndr

Entrez Gene IDs

4881 (Human); 18160 (Mouse); 24603 (Rat)

Gene Symbol

NPR1

UniProt

Additional NPRA/NPR1 Products

Product Documents for Human NPRA/NPR1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human NPRA/NPR1 Antibody

For research use only

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