Human Notum Alexa Fluor™ Plus 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB9356AFP488

Catalog #
Availability
Size / Price
Qty
FAB9356AFP488-100UG
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 962216

View all Notum Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Notum

Specificity

Detects human Notum in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human Notum Alexa Fluor™ Plus 488-conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Notum

Notum is an evolutionarily conserved 60 kDa secreted deacylase that regulates Wnt activity (1, 2). It is tethered to the cell surface by binding to Glypican-like sulfated proteoglycans, and it functions as a Glypican-dependent Wnt inhibitor (1-4). Notum hydrolyzes the palmitoyl moiety from Wnt-3a, making the Wnt more hydrophilic and unable to bind to the Frizzled-8 receptor (1, 2). It also induces the release of cell surface GPI-anchored proteins such as Glypican-3, Cadherin-13, and uPAR (4). Notum regulates head regeneration in planaria (3), head formation and neural induction in Xenopus (1), and long range activity of hedgehog proteins in Drosophila (5). It is up-regulated in some human hepatocellular carcinomas and colorectal cancers (6, 7). Within amino acids
81-451, human Notum shares 92% aa sequence identity with mouse and rat Notum.

References

  1. Zhang, X. et al. (2015) Dev. Cell 32:719.
  2. Kakugawa, S. et al. (2015) Nature 519:187.
  3. Petersen, C.P. and P.W. Reddien (2011) Science 332:852.
  4. Traister, A. et al. (2008) Biochem. J. 410:503.
  5. Ayers, K.L. et al. (2010) Dev. Cell 18:605.
  6. Torisu, Y. et al. (2008) Cancer Sci. 99:1139.
  7. De Robertis, M. et al. (2015) Oncotarget 6:41237.

Alternate Names

EC 3.-, notum pectinacetylesterase homolog (Drosophila), protein notum homolog

Entrez Gene IDs

147111 (Human); 77583 (Mouse); 303743 (Rat)

Gene Symbol

NOTUM

UniProt

Q6P988

Additional Notum Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Notum Alexa Fluor™ Plus 488-conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Privacy Policy
Cookie Policy
Site Map
© Copyright 2026 Bio-Techne. All Rights Reserved. All trademarks and registered trademarks are the property of Bio-Techne and its brands unless otherwise specified.