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Human Niemann-Pick Type C2/NPC2 Antibody

Catalog # AF8644 | R&D Systems, Inc. a Bio-Techne Brand
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AF8644
AF8644-SP

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Product Summary for Human Niemann-Pick Type C2/NPC2 Antibody

Immunogen

S. frugiperda insect ovarian cell line Sf21-derived recombinant human Niemann-Pick Type C2/NPC2
Glu20-Leu151
Accession # P61916

Specificity

Detects human Niemann-Pick Type C2/NPC2 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Niemann-Pick Type C2/NPC2 Antibody

Detection of Human Niemann-Pick Type C2/NPC2 antibody by Western Blot.

Detection of Human Niemann-Pick Type C2/NPC2 by Western Blot.

Western blot shows lysates of NTera-2 human testicular embryonic carcinoma cell line, human kidney (cortex) tissue, HepG2 human hepatocellular carcinoma cell line, and human skeletal muscle tissue. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human Niemann-Pick Type C2/NPC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8644) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Niemann-Pick Type C2/NPC2 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Niemann-Pick Type C2/NPC2 antibody in SK-Hep-1 Human Cell Line by Immunocytochemistry (ICC).

Niemann-Pick Type C2/NPC2 in SK-Hep-1 Human Cell Line.

Niemann-Pick Type C2/NPC2 was detected in immersion fixed SK-Hep-1 human liver adenocarcinoma cell line using Rabbit Anti-Human Niemann-Pick Type C2/NPC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8644) at 1:150 dilution for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Niemann-Pick Type C2/NPC2 antibody in Human Kidney by Immunohistochemistry (IHC-P).

Niemann-Pick Type C2/NPC2 in Human Kidney.

Niemann-Pick Type C2/NPC2 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human Niemann-Pick Type C2/NPC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8644) at 1:50 dilution overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in tubular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Niemann-Pick Type C2/NPC2 Antibody

Application
Recommended Usage

Immunocytochemistry

1:150 dilution
Sample: Immersion fixed SK-Hep-1 human liver adenocarcinoma cell line

Immunohistochemistry

1:50 dilution
Sample: Immersion fixed paraffin-embedded sections of human kidney

Western Blot

1:1000 dilution
Sample: NTera‑2 human testicular embryonic carcinoma cell line, human kidney (cortex) tissue, and HepG2 human hepatocellular carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using AF8644 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

  • 12 months from date of receipt, -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 °C under sterile conditions after opening.

Background: Niemann-Pick Type C2/NPC2

Alternate Names

EDDM1, Epididymal Protein 1, HE1, NiemannPick Type C2, NPC2

Entrez Gene IDs

10577 (Human); 67963 (Mouse); 286898 (Rat)

Gene Symbol

NPC2

UniProt

Product Documents for Human Niemann-Pick Type C2/NPC2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Niemann-Pick Type C2/NPC2 Antibody

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.

For research use only

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FAQs for Human Niemann-Pick Type C2/NPC2 Antibody

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  • Q: I'm looking for a Niemann-Pick type1 or 2 antibody to stain mouse heart section embedded in paraffin. I would like to label these sections with another primary antibody to show colocalisation of my protein of interest. I would like to know if you have a rabbit anti-Niemann-Pick antibody that works well for fluorescence immunohistochemistry?

    A: Niemann-Pick C1 Antibody (NB400-148) (0.1 ml) is one of our best sellers and this antibody has been cited in at least 17 peer reviewed research publications in journals of high repute. We have only one Niemann-Pick type C2 antibody with catalog # H00010577-D01PInnovators Reward Program.

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