Human Niemann-Pick Type C2/NPC2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF8644
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot, Immunocytochemistry
Cited:
Western Blot, Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf21-derived recombinant human Niemann-Pick Type C2/NPC2
Glu20-Leu151
Accession # P61916
Glu20-Leu151
Accession # P61916
Specificity
Detects human Niemann-Pick Type C2/NPC2 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Niemann-Pick Type C2/NPC2 Antibody
Detection of Human Niemann-Pick Type C2/NPC2 by Western Blot.
Western blot shows lysates of NTera-2 human testicular embryonic carcinoma cell line, human kidney (cortex) tissue, HepG2 human hepatocellular carcinoma cell line, and human skeletal muscle tissue. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human Niemann-Pick Type C2/NPC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8644) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Niemann-Pick Type C2/NPC2 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Niemann-Pick Type C2/NPC2 in SK-Hep-1 Human Cell Line.
Niemann-Pick Type C2/NPC2 was detected in immersion fixed SK-Hep-1 human liver adenocarcinoma cell line using Rabbit Anti-Human Niemann-Pick Type C2/NPC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8644) at 1:150 dilution for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Niemann-Pick Type C2/NPC2 in Human Kidney.
Niemann-Pick Type C2/NPC2 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human Niemann-Pick Type C2/NPC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8644) at 1:50 dilution overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in tubular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Niemann-Pick Type C2/NPC2 Antibody
Application
Recommended Usage
Immunocytochemistry
1:150 dilution
Sample: Immersion fixed SK-Hep-1 human liver adenocarcinoma cell line
Sample: Immersion fixed SK-Hep-1 human liver adenocarcinoma cell line
Immunohistochemistry
1:50 dilution
Sample: Immersion fixed paraffin-embedded sections of human kidney
Sample: Immersion fixed paraffin-embedded sections of human kidney
Western Blot
1:1000 dilution
Sample: NTera‑2 human testicular embryonic carcinoma cell line, human kidney (cortex) tissue, and HepG2 human hepatocellular carcinoma cell line
Sample: NTera‑2 human testicular embryonic carcinoma cell line, human kidney (cortex) tissue, and HepG2 human hepatocellular carcinoma cell line
Reviewed Applications
Read 1 review rated 4 using AF8644 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
For liquid material, refer to CoA for concentration.
Formulation
Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
- 12 months from date of receipt, -20 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
- 6 months, -20 °C under sterile conditions after opening.
Background: Niemann-Pick Type C2/NPC2
Alternate Names
EDDM1, Epididymal Protein 1, HE1, NiemannPick Type C2, NPC2
Gene Symbol
NPC2
UniProt
Additional Niemann-Pick Type C2/NPC2 Products
Product Documents for Human Niemann-Pick Type C2/NPC2 Antibody
Product Specific Notices for Human Niemann-Pick Type C2/NPC2 Antibody
* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.
For research use only
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