Human Neuropilin-1 Antibody

Name: Human Neuropilin-1 Antibody
Brand: R&D Systems
SKU: MAB3870
Price: 160 EUR

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB3870

Citations (3)

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Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human Neuropilin‑1 Phe22-Lys644 Accession # NP_001019799
Applications:	CyTOF-ready, Flow Cytometry
Label:	Unconjugated
Source:	Monoclonal Mouse IgG_2A Clone # 446921
Specificity:	Detects human Neuropilin-1 in direct ELISAs.

View Full Technical Details for Human Neuropilin-1 Antibody

Applications for Human Neuropilin-1 Antibody

	Recommended Concentration	Sample
Flow Cytometry	2.5 µg/10⁶ cells	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human Neuropilin-1 Antibody

	Flow Cytometry Detection of Neuropilin‑1 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Human Neuropilin-1 Monoclonal Antibody (Catalog # MAB3870, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B).

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Neuropilin-1

Neuropilin-1 (Npn-1, previously neuropilin; also CD304) is a 130‑140 kDa type I transmembrane (TM) glycoprotein that regulates axon guidance and angiogenesis (1‑4). The full-length 923 amino acid (aa) human Npn‑1 contains a 623 aa extracellular domain (ECD) that shows 92‑95% aa identity with mouse, rat, bovine and canine
Npn‑1 (3, 4). The ECD contains two N-terminal CUB domains (termed a1a2), two domains with homology to coagulation factors V and VIII (b1b2) and a MAM (meprin) domain (c). C-terminally divergent splice variants with 704, 644, 609, and 551 aa lack the MAM and TM domains and are demonstrated or presumed to be soluble antagonists (1, 5‑7). A 906 aa form lacks a TM segment, but secretion has not been found (8). The sema domains of Class III secreted semaphorins such as Sema3A bind Npn-1 a1a2 (9). Heparin, the heparin-binding forms of VEGF (VEGF₁₆₅, VEGF-B and VEGF-E), PlGF (PlGF2), and the C-terminus of Sema3 bind the b1b2 region (9, 10). Npn-1 and Npn-2 share 48% aa identity within the ECD and can form homo- and hetero-oligomers via interaction of their MAM domains (1). Neuropilins show partially overlapping expression in neuronal and endothelial cells during development (1, 2). Both neuropilins act as co‑receptors with plexins, mainly plexin A3 and A4, to bind class III semaphorins that mediate axon repulsion (11). However, only Npn-1 binds Sema3A, and only Npn-2 binds Sema3F (1). Both are co-receptors with VEGF R2 (also called KDR or Flk-1) for VEGF₁₆₅ binding (1). Sema3A signaling can be blocked by VEGF₁₆₅, which has higher affinity for Npn-1 (12). Npn-1 is preferentially expressed in arteries during development or those undergoing remodeling (1, 2). Npn-1 is also expressed on dendritic cells and mediates DC-induced T cell proliferation (13).

Item	Value
References	Bielenberg, D.R. et al. (2006) Exp. Cell Res. 312:584. Gu, C. et al. (2003) Dev. Cell 5:45. He, Z. and M. Tessier-Lavigne (1997) Cell 90:739. Soker, S. et al. (1998) Cell 92:735. Gagnon, M.L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:2573. Cackowski, F.C. et al. (2004) Genomics 84:82. Rossignol, M. et al. (2000) Genomics 70:211. Tao, Q. et al. (2003) Angiogenesis 6:39. Gu, C. et al. (2002) J. Biol. Chem. 277:18069. Mamluk, R. et al. (2002) J. Biol. Chem. 277:24818. Yaron, A. et al. (2005) Neuron 45:513. Narazaki, M. and G. Tosato (2006) Blood 107:3892. Tordjman, R. et al. (2002) Nat. Immunol. 3477.
Entrez Gene IDs	8829 (Human); 18186 (Mouse); 246331 (Rat); 100286859 (Porcine)
Alternate Names	BDCA-4, BDCA4, CD304, CD304 antigen, DKFZp686A03134, DKFZp781F1414, NP1, NRP, NRP1, Neuropilin-1, Neuropilin1, VEGF165R, Vascular endothelial cell growth factor 165 receptor, neuropilin 1, transmembrane receptor

Citations for Human Neuropilin-1 Antibody (3)

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Citations are publications that use Bio-Techne products. Selected citations for Human Neuropilin-1 Antibody include:

Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation

N Gioelli et al. (2022), Neuropilin 1 and its inhibitory ligand mini-tryptophanyl-tRNA synthetase inversely regulate VE-cadherin turnover and vascular permeability Nature Communications, 13(1):4188.
PMID: 35858913
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

JR Podojil et al. (2018), B7-H4 Modulates Regulatory CD4+ T Cell Induction and Function via Ligation of a Semaphorin 3a/Plexin A4/Neuropilin-1 Complex J. Immunol., 0(0).
PMID: 29898965
Species: Mouse
Sample Types: Protein
Applications: Neutralization

Jaalouk DE et al. (2007), The original Pathologische Anatomie Leiden-Endothelium monoclonal antibody recognizes a vascular endothelial growth factor binding site within neuropilin-1. Cancer Res., 67(20):9623-9.
PMID: 17942890

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Human Neuropilin-1 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human Neuropilin-1 Antibody

Scientific Data Examples for Human Neuropilin-1 Antibody

Flow Cytometry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Neuropilin-1

Citations for Human Neuropilin-1 Antibody (3)

Product Documents for Human Neuropilin-1 Antibody

Product Datasheets

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