Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all NDRG1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NDRG1
Met111-Asp267
Accession # Q92597

Specificity

Detects human NDRG1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human N-myc is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human NDRG1 Antibody

Detection of Human NDRG1 antibody by Western Blot.

Detection of Human NDRG1 by Western Blot.

Western blot shows lysates of human liver tissue and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human NDRG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5209) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for NDRG1 at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human NDRG1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human liver tissue and HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NDRG1

NDRG1 (N-myc downstream-regulated gene 1; also DRG-1 and Cap43) is a 43 kDa member of the NDRG family of proteins. It is ubiquitously expressed, found in fibroblasts, endothelial cells, respiratory epithelium and prostate epithelium. NDRG1 is highly expressed in nonproliferating, differentiating tissue. Human NDRG1 is 394 amino acids (aa) in length. It contains an NDGR domain (aa 286‑316) plus three tandem 10 aa hydrophilic repeats (aa 339‑368). There are three potential isoform variants. Two involve alternate start sites at Met82 and Met286, while a third shows a four aa substitution for aa 1‑34. Over aa 11‑267, human NDRG1 is 95% aa identical to mouse NDRG1.

Long Name

N-myc Downstream Regulated Gene 1

Alternate Names

CAP43, CMT4D, DRG1, GC4, HMSNL, NMSL, PROXY1, RIT42, RTP, TARG1, TDD5

Entrez Gene IDs

10397 (Human); 17988 (Mouse); 299923 (Rat)

Gene Symbol

NDRG1

UniProt

Q92597

Additional NDRG1 Products

Product Documents for Human NDRG1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human NDRG1 Antibody

For research use only

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