MyoD was detected in fixed C2C12 mouse myoblast cell line (Positive) and absent in RD cells (Negative) using Mouse Anti-Human MyoD Monoclonal Antibody (Catalog # MAB5966) at 25 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of MyoD by Western Blot

Cdkn1c is required for the proper formation of iBAT.(A) Photograph of E18.5 WT and Cdkn1c-/+ (KOMAT) fetuses with position of iBAT depot highlighted by dotted black line. (B) H&E staining of iBAT sections of E18.5 WT and KOMAT iBAT. (C) QPCR of Cdkn1c and the adipocyte regulators Rb1, PPAR gamma, C/EBP alpha and C/EBP beta, pan-adipocyte genes Fabp4, Cidec and Plin1, BAT-selective genes Ppargc1a, Cidea, Prdm16, Ucp1 and Elovl3, mitochondrial genes Cycs and Cox2, and the skeletal muscle-selective genes Myf5 and Myod1 in E18.5 KOMAT iBAT relative to WT (n = 4 per genotype). (D) Mitochondrial DNA content of iBAT from E18.5 KOMAT iBAT relative to WT (n = 6 per genotype). (E) Western blot analysis of UCP1 and beta-ACTIN in E18.5 iBAT isolated from two WT and two KOMAT fetuses. Within litter comparison. (F) Western blot analysis of CDKN1C, PRDM16 and beta-ACTIN in E18.5 iBAT isolated from two WT and two KOMAT fetuses. Within litter comparison. (G) Western blot analysis of MYOD and GAPDH in E18.5 E18.5 iBAT isolated from two WT and two KOMAT fetuses. Within litter comparison. Data expressed as mean ± SEM, t test. * P <0.05; ** P <0.01; *** P <0.005. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26963625), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human MyoD Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: fixed C2C12 mouse myoblast cell line (Positive) and absent in RD cells (Negative)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MyoD

MyoD (myoblast determination protein 1), also called Myf-3 (myogenic factor 3) is an ~44 kDa nuclear protein in the MyoD family of muscle-specific bHLH transcription factors. MyoD family members heterodimerize with E proteins and cooperate with MEF2 family transcription factors to regulate expression of skeletal muscle-specific genes. MyoD is essential for skeletal muscle differentiation. Acetylation at lysines 99, 102 and 104 further regulates its activity. Human MyoD shares 96% amino acid identity with mouse and rat MyoD over the sequence used as the immunogen.

Long Name

Myoblast Determination Protein 1

Alternate Names

bHLHc1, MYF3, MYOD1, PUM

Entrez Gene IDs

4654 (Human); 17927 (Mouse); 337868 (Rat)

Gene Symbol

MYOD1

UniProt

P15172

Additional MyoD Products

Product Documents for Human MyoD Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human MyoD Antibody

For research use only

Citations
Reviews