Human/Mouse TRAP220 Alexa Fluor™ Plus 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5520AFP488
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Specificity
Detects endogenous human TRAP220 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Background: TRAP220
TRAP220 (Thyroid hormone receptor associated protein 220 kDa) is a widely expressed member of the Mediator complex subunit 1 family of proteins. It constitutes one of some 25 subunits that make up the Mediator complex. Here, it serves as a scaffold protein that links nuclear hormone receptors to RNA polymerase II. Human TRAP220 is 1581 amino acids (aa) in length. It contains a Mediator complex interaction region (aa 108‑390) and two nuclear hormone receptor binding boxes (aa 600‑670). Phosphorylation of Thr1032 and Thr1457 materially increases TRAP220 activity. There are at least two splice variants. One shows a 22 aa substitution for aa 756‑1581 while another shows a nine aa substitution for aa 548‑1581. Over aa 878‑1031, human TRAP220 shares 92% aa identity with mouse TRAP220.
Long Name
Thyroid Hormone Receptor-associated Protein Complex Component 220 kDa
Alternate Names
DRIP230, MBD4, MED1, PPARBP, PPARGBP, TRIP2
Gene Symbol
MED1
UniProt
Additional TRAP220 Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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