Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse THAP11 Monoclonal Antibody (Catalog # MAB5727) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for THAP11 at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

THAP11 in BG01V Human Stem Cells.

THAP11 was detected in immersion fixed undifferentiated BG01V human embryonic stem cells using Mouse Anti-Human/Mouse THAP11 Monoclonal Antibody (Catalog # MAB5727) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human THAP11 by Simple Western^TM.

Simple Western lane view shows lysates of BG01V human embryonic stem cells, loaded at 0.2 mg/mL. A specific band was detected for THAP11 at approximately 56 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse THAP11 Monoclonal Antibody (Catalog # MAB5727). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse THAP11 Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed undifferentiated BG01V human embryonic stem cells

Simple Western

20 µg/mL
Sample: BG01V human embryonic stem cells

Western Blot

2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: THAP11

THAP11, also known as Ronin, is a 36-44 kDa member of the thanatos-associated protein family. It is ubiquitously expressed and through its repression of c-Myc transcription, blocks cell proliferation. Human THAP11 is 314 amino acids (aa) in length. It contains a DNA-binding THAP zinc-finger domain (aa 5-81), an NLS (aa 98-104), a polyGln region (aa 104-146), an Ala-rich segment (aa 197-221) and a C-terminal coiled-coil region (aa 255-305). THAP11 is phosphorylated on Thr25. Over aa 226-314, human and mouse THAP11 are 100% identical in amino acid sequence.

Long Name

THAP Domain Containing 11

Alternate Names

CTG-B43a, CTG-B45d, Ronin

Entrez Gene IDs

57215 (Human); 59016 (Mouse); 307806 (Rat)

Gene Symbol

THAP11

UniProt

Q96EK4

Additional THAP11 Products

Product Documents for Human/Mouse THAP11 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse THAP11 Antibody

For research use only

Citations
Reviews

Human/Mouse THAP11 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5727

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all THAP11 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse THAP11 Antibody

Detection of Human THAP11 by Western Blot.