Western blot shows lysates of A20 mouse B cell lymphoma cell line, DA3 mouse myeloma cell line, Daudi human Burkitt's lymphoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Human/Mouse TANK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4755) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TANK at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse TANK Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: A20 mouse B cell lymphoma cell line, DA3 mouse myeloma cell line, Daudi human Burkitt's lymphoma cell line, and Raji human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TANK

TRAF (tumor necrosis factor receptor-associated factor) family member-associated NF-kappaB activator (TANK) maintains TRAF1, TRAF2 and TRAF3 proteins in their latent states (cytoplasmic sequestration). TANK binds to their TRAF-c domains inhibiting TRAF signaling and NF kappaB activation. TANK may function as an adaptor molecule in multiple antiviral pathways. Two transcript variants encoding different isoforms for this gene have been found.

Long Name

TRAF Family Member-Associated NFKB Activator

Alternate Names

I-TRAF

Entrez Gene IDs

10010 (Human); 21353 (Mouse); 252961 (Rat)

Gene Symbol

TANK

UniProt

P70347

Additional TANK Products

Product Documents for Human/Mouse TANK Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse TANK Antibody

For research use only

Citations
Reviews

Human/Mouse TANK Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4755

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse TANK Antibody

Detection of Human/Mouse TANK by Western Blot.