Human/Mouse STAT6 Antibody
R&D Systems, part of Bio-Techne | Catalog # PA-ST6
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Western Blot, Immunoprecipitation
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit Serum
Product Specifications
Specificity
Detects human and murine STAT 6. This antiserum does not crossreact with any of the other STAT proteins.
Clonality
Polyclonal
Host
Rabbit
Isotype
Serum
Scientific Data Images for Human/Mouse STAT6 Antibody
Detection of Human STAT6 by Western Blot.
Western blot shows lysates of TF-1 human erythroleukemic cell line and human peripheral blood lymphocyte. PVDF membrane was probed with 1:2000 dilution of Human/Mouse STAT6 Polyclonal Antibody (Catalog # PA-ST6) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for STAT6 at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 9.Applications for Human/Mouse STAT6 Antibody
Application
Recommended Usage
Immunoprecipitation
3-6 µg/106 cells
Sample: TF-1 human erythroleukemic cell line, see our available Western blot detection antibodies
Sample: TF-1 human erythroleukemic cell line, see our available Western blot detection antibodies
Western Blot
2000x µg/mL
Sample: TF-1 human erythroleukemic cell line and human peripheral blood lymphocyte
Sample: TF-1 human erythroleukemic cell line and human peripheral blood lymphocyte
Formulation, Preparation, and Storage
Formulation
Lyophilized from 0.2 mL antiserum containing 0.02% sodium azide
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: STAT6
Signal Transducer and Activator of Transcription 6 (STAT6) is a member of the Jak/STAT signal transduction pathway and mediates cytokine signaling by IL-4. IL-4 activation leads to tyrosine phosphorylation and accumulation in the nucleus of STAT6 homo and heterodimers. STAT6 acts as a signal transducer in the cytoplasm and, upon phosphorylation at Y641, translocates to the nucleus and binds to the DNA consensus site TTCN4GAA. Knockout studies in mice suggest that STAT6 functions in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. STAT6 transcriptional activation occurs by binding p100 and p160 class (NcoA1, Src-1) co-activators. Gene expression studies indicate that STAT6 mediates cell proliferation and anti-apoptotic effects of IL-4.
Long Name
Signal Transducer and Activator of Transcription 6
Alternate Names
D12S1644, EC 2.4.1.227, EC 2.7.7.6, IL-4 Stat, IL-4-STAT, signal transducer and activator of transcription 6, signal transducer and activator of transcription 6, interleukin-4 induced, STAT, interleukin-4 induced, STAT, interleukin4-induced, STAT6B, STAT6C, transcription factor IL-4 STAT
Gene Symbol
STAT6
Additional STAT6 Products
Product Documents for Human/Mouse STAT6 Antibody
Product Specific Notices for Human/Mouse STAT6 Antibody
For research use only
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