Human/Mouse/Rat TOR Antibody
R&D Systems, part of Bio-Techne | Catalog # AF15371
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse, Rat
Applications
Validated:
Western Blot, Immunoprecipitation
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human TOR
Phe1720-Ala2020
Accession # P42345
Phe1720-Ala2020
Accession # P42345
Specificity
Detects human, mouse, and rat TOR.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat TOR Antibody
Detection of Human/Mouse/Rat TOR by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line, Balb/3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat TOR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF15371) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TOR at approximately 280 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat TOR Antibody
Application
Recommended Usage
Immunoprecipitation
2 µg/500 µg cell lysate
Sample: MCF-7 human breast cancer cell line, see our available Western blot detection antibodies
Sample: MCF-7 human breast cancer cell line, see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: NRK rat normal kidney cell, MCF-7 human breast cancer cell line, and Balb/3T3 mouse embryonic fibroblast cell line
Sample: NRK rat normal kidney cell, MCF-7 human breast cancer cell line, and Balb/3T3 mouse embryonic fibroblast cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TOR
The Target of Rapamycin (TOR) is a member of the PI 3-kinase-related kinase (PIKK) family. TOR is the protein target of rapamycin, an anti-rejection drug used in transplantation and promising anti-cancer agent. TOR plays a crucial role in the control of cell growth and proliferation as a downstream target of the PI 3-kinase/Akt signal transduction pathway.
Long Name
Mammalian Target of Rapamycin
Alternate Names
FRAP1, FRAP2, MTOR, RAFT1
Gene Symbol
MTOR
UniProt
Additional TOR Products
Product Documents for Human/Mouse/Rat TOR Antibody
Product Specific Notices for Human/Mouse/Rat TOR Antibody
For research use only
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