Human/Mouse/Rat Thioredoxin-2 Alexa Fluor™ Plus 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3254AFP488
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse Thioredoxin-2
Specificity
Detects endogenous human, mouse and rat Thioredoxin 2 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Thioredoxin-2
Thioredoxin-2 (Trx2), also known as mitochondrial Thioredoxin, belongs to the evolutionarily conserved Thioredoxin family. These proteins share the Thioredoxin fold containing the active site-CGPC motif. In their reduced form, the active site cysteine residues reduce protein disulfides. The resulting active site disulfide is subsequently reduced in a reaction catalyzed by a NADPH-dependent Thioredoxin reductase. Thioredoxin-2 contains an N-terminal 59 amino acid (aa) transit sequence that is cleaved upon translocation to mitochondria. The amino acid sequence of mature mouse Thioredoxin‑2 is identical to mature rat Thioredoxin-2 and 98% identical to human Thioredoxin-2. Thioredoxin-2 interacts with specific components of the mitochondrial respiratory chain and helps regulate the membrane potential. Thioredoxin-2 is ubiquitously but variably expressed and high expression confers resistance to oxidant‑induced apoptosis.
Alternate Names
MT-TRX, Thioredoxin2, Trx2, TXN2
Gene Symbol
TXN2
UniProt
Additional Thioredoxin-2 Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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