Western blot shows lysates of NRK rat normal kidney cell line, Ramos human Burkitt's lymphoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Rheb Monoclonal Antibody (Catalog # MAB3426) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for Rheb at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Rheb in Ramos Human Cell Line.

Rheb was detected in immersion fixed Ramos human Burkitt's lymphoma cell line using Mouse Anti-Human/Mouse/Rat Rheb Monoclonal Antibody (Catalog # MAB3426) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Rheb in NIH-3T3 mouse embryonic fibroblast cell line (Positive) & PC-12 rat adrenal pheochromocytoma cell line (Negative).

Rheb was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line (Positive) & PC-12 rat adrenal pheochromocytoma cell line (Negative) using Mouse Anti-Human/Mouse/Rat Rheb Monoclonal Antibody (Catalog # MAB3426) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to Cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Mouse/Rat Rheb Antibody

Application

Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed Ramos human Burkitt's lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line (Positive) & PC-12 rat adrenal pheochromocytoma cell line (Negative)

Western Blot

1 µg/mL
Sample: NRK rat normal kidney cell, Ramos human Burkitt's lymphoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Rheb

Ras homolog enriched in brain (Rheb) is a novel member of the Ras superfamily of GTP-binding proteins involved in cell growth and cell cycle progression. Expressed in a wide range of tissues, Rheb was initially identified from its induction in rat hippocampal granule cells by seizures and NMDA-dependent synaptic activity. A complex containing the tuberous sclerosis gene products TSC1 and TSC2 functions as a GTPase-activating protein (GAP) for Rheb.

Long Name

Ras Homolog Enriched In Brain

Alternate Names

Rheb2

Entrez Gene IDs

6009 (Human); 19744 (Mouse); 26954 (Rat)

Gene Symbol

RHEB

UniProt

AAH66307

Additional Rheb Products

Product Documents for Human/Mouse/Rat Rheb Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human/Mouse/Rat Rheb Antibody

For research use only

Citations
Reviews

Human/Mouse/Rat Rheb Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3426

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Rheb Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse/Rat Rheb Antibody

Detection of Human/Mouse/Rat Rheb by Western Blot.