Human/Mouse/Rat Ras-GAP Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5094
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Ras-GAP
Trp181-Val272
Accession # P20936
Trp181-Val272
Accession # P20936
Specificity
Detects endogenous human, mouse, and rat Ras-GAP in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Ras-GAP Antibody
Detection of Human/Mouse/Rat Ras-GAP by Western Blot.
Western blot shows lysates of TT human medullary thyroid cancer cell line, C2C12 mouse myoblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Ras-GAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5094) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Ras-GAP at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat Ras-GAP Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: TT human medullary thyroid cancer cell line, C2C12 mouse myoblast cell line, and Rat-2 rat embryonic fibroblast cell line
Sample: TT human medullary thyroid cancer cell line, C2C12 mouse myoblast cell line, and Rat-2 rat embryonic fibroblast cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ras-GAP
Ras-GAP (Ras GTPase-activating protein) interacts with Ras to stimulate the otherwise weak intrinsic GTPase activity of Ras and to promote the return of Ras to an inactive GDP-bound state. Thus, Ras-GAP is a negative regulator of Ras. Human Ras-GAP is 1047 amino acids in length. The N-terminal region contains two SH2 domains and an intervening SH3 domain, while the C-terminal half of Ras-GAP is sufficient to accelerate Ras GTPase activity.
Long Name
GTPase activating protein
Alternate Names
CM-AVM, PKWS, RASA1, RasGAP
Gene Symbol
RASA1
UniProt
Additional Ras-GAP Products
Product Documents for Human/Mouse/Rat Ras-GAP Antibody
Product Specific Notices for Human/Mouse/Rat Ras-GAP Antibody
For research use only
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