Detects endogenous human, mouse and rat PRL-3 in Western blots. In Western blots, this antibody does not cross‑react with recombinant human PRL-1 or PRL‑2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human/Mouse/Rat PRL-3 Antibody

Detection of Human/Mouse/Rat PRL‑3 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Neuro-2A mouse neuroblastoma cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 0.3 µg/mL of Human/Mouse/Rat PRL-3 Monoclonal Antibody (Catalog # MAB3219) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PRL-3 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat PRL-3 Antibody

Application

Recommended Usage

Western Blot

0.3 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, Neuro-2A mouse neuroblastoma cell line, and Nb2-11 rat lymphoma cell line

Reviewed Applications

Read 3 reviews rated 3.7 using MAB3219 in the following applications:

ELISA (1 Review)
Microarray (1 Review)
Microarrays (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PRL-3/PTP4A3

Phosphatase of Regenerating Liver-3 (PRL-3), also known as PTP4A3, is an enzyme that removes phosphate modifications from tyrosine residues on proteins. Prenylation at the C-terminal end targets PRL-3 to membrane compartments in the cell, especially to early endosomes and the plasma membrane. Elevated levels of PRL-3 are observed in metastatic colorectal, ovarian, liver and skin cancers. PRL-3 levels may correlate with cell migration rates and tumor invasiveness.

Long Name

Phosphatase of Regenerating Liver 3

Alternate Names

PRL3, PTP4A3

Entrez Gene IDs

11156 (Human); 19245 (Mouse)

Gene Symbol

PTP4A3

UniProt

O75365

Additional PRL-3/PTP4A3 Products

Product Documents for Human/Mouse/Rat PRL-3 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse/Rat PRL-3 Antibody

For research use only

Citations
Reviews

Human/Mouse/Rat PRL-3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3219

Key Product Details

Species Reactivity

Validated:

Cited:

Applications