Human/Mouse/Rat PP2C alpha/PPM1A Alexa Fluor™ Plus 405-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB4150AFP405
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Alexa Fluor Plus 405 (Excitation = 404 nm, Emission = 455 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 498922
Product Specifications
Immunogen
E. coli-derived recombinant human PP2C alpha/PPM1A
Specificity
Detects human, mouse, and rat PP2C alpha/PPM1A in Western blots. No cross-reactivity with recombinant human PP2C beta, ILKAP/PP2C delta, or PP2C gamma is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: PP2C alpha/PPM1A
Protein phosphatase 2C alpha, also called PP2C alpha, Protein Phosphatase, Magnesium-dependent type 1A, and PPM1A, dephosphorylates proteins with some preference for phospho-threonine over phospho-serine residues (1). It is insensitive to the PP1 and PP2a inhibitors such as okadaic acid, (2) but has an absolute requirement for either magnesium or manganese ions (3) and can be activated by unsaturated fatty acids such as arachidonic and oleic acids (4). Overexpression of PP2C alpha causes G2/M cell cycle arrest and apoptosis that has been associated with the activation of p53 (5). Dephosphorylation of proteins in the nucleus by PP2C alpha is suspected to play a role in terminating or reducing the sensitivity of the responses to SMADS (6) and stress-activated proteins such as p38 and JNK (7).
References
- Donella-Deanna, A. et al. (1991) Biochimica et Biophysica Acta 1094:130.
- Bialojan, C. and A. Takai (1998) Biochem. J. 256:283.
- Cohen, P. Annu. Rev. Biochem. (1989) 58:453.
- Klumpp, S. et al. (1998) FEBS Let. 437:229.
- Ofek, P. et al. (2003) J. Biol. Chem. 278:14299.
- Lin, X. et al. (2006) Cell 125:915.
- Takekawa, M. et al. (1998) EMBO J. 17:4744.
Long Name
Protein Phosphatase 2C alpha
Alternate Names
MMPa-2, MPPa-1, Pp2c1, PPM1A
Gene Symbol
PPM1A
UniProt
Additional PP2C alpha/PPM1A Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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