Human/Mouse/Rat PP2A Catalytic Subunit Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1653
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PP2A-alpha catalytic subunit
Trp209-Leu309
Accession # P67775
Trp209-Leu309
Accession # P67775
Specificity
Detects endogenous human, mouse, and rat PP2A in Western blots. Because PP2A-alpha and PP2A-beta catalytic subunits are identical in the region used for the antigen, it is expected that both isoforms will be detected by the antibody.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat PP2A Catalytic Subunit Antibody
Detection of Human/Mouse/Rat PP2A Catalytic Subunit, by Western Blot.
Western blot shows lysates of U937 human histiocytic lymphoma cell line, C2C12 mouse myoblast cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Human/Mouse/Rat PP2A Catalytic Subunit, Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1653) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PP2A Catalytic Subunit, at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat PP2A Catalytic Subunit Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: U937 human histiocytic lymphoma cell line, C2C12 mouse myoblast cell line, and Nb2-11 rat lymphoma cell line
Sample: U937 human histiocytic lymphoma cell line, C2C12 mouse myoblast cell line, and Nb2-11 rat lymphoma cell line
Reviewed Applications
Read 1 review rated 4 using AF1653 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PP2A
Protein Phosphatase 2A (PP2A) is an enzyme that removes phosphates covalently attached to serine and threonine residues in proteins. It contains three subunits; a catalytic subunit C (PP2Ac), an assembly/structural subunit A (PP2Aa), and a regulatory subunit B (PP2Ab). PP2A is a ubiquitous enzyme that plays a role in regulating many cellular activities varying from ion transport in erythrocytes to suppressing tumor growth. Binding of the SV40 small T antigen to the PP2A phosphatase complex is known to cause oncogenic transformation.
Alternate Names
PP2CA, PPP2CA, RP-C
Gene Symbol
PPP2CA
UniProt
Additional PP2A Products
Product Documents for Human/Mouse/Rat PP2A Catalytic Subunit Antibody
Product Specific Notices for Human/Mouse/Rat PP2A Catalytic Subunit Antibody
For research use only
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