Detects human, mouse and rat PP1 Inhibitor-2 in Western blots. In Western blots, no cross-reactivity with recombinant human PP1 Inhibitor-1 or DARPP-32 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat PP1 Inhibitor-2 Antibody

Detection of Human/Mouse/Rat PP1 Inhibitor-2 by Western Blot.

Western blot shows lysates of Raji human Burkitt's lymphoma cell line and mouse spleen tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat PP1 Inhibitor-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4719) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PP1 Inhibitor-2 at approximately 30 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Detection of Human PP1 Inhibitor-2 by Simple Western^TM.

Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PP1 Inhibitor-2 at approximately 42 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat PP1 Inhibitor-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4719) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse/Rat PP1 Inhibitor-2 Antibody

Application

Recommended Usage

Simple Western

10 µg/mL
Sample: Raji human Burkitt's lymphoma cell line

Western Blot

1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line and mouse spleen tissue

Reviewed Applications

Read 2 reviews rated 4.5 using AF4719 in the following applications:

Western Blot (2 Reviews)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PP1 Inhibitor-2

Protein Phosphatase 1 Inhibitor-2 (PP1 Inhibitor-2), also called PP1 regulatory subunit 2 (PP1R2), I2, and IPP2, binds to PP1 through an RVxF consensus sequence. The complex inactivates PP1, preventing it from dephosphorylating substrates such as glycogen phosphorylase. Unlike PP1 Inhibitor-1, the binding of PP1 Inhibitor-2 is Protein Kinase A phosphorylation-independent. Dissociation of the PP1 Inhibitor-2 complex is enhanced by phosphorylation at T72 by GSK3, ERK, or CDK. Overexpression of PP1 Inhibitor-2 in mouse heart improves cardiac function without changing organ size.

Long Name

Protein Phosphatase 1 Inhibitor 2

Alternate Names

IPP2, PP1 Inhibitor2, PPP1R2

Entrez Gene IDs

5504 (Human); 66849 (Mouse); 192361 (Rat)

Gene Symbol

PPP1R2

UniProt

P41236

Additional PP1 Inhibitor-2 Products

Product Documents for Human/Mouse/Rat PP1 Inhibitor-2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse/Rat PP1 Inhibitor-2 Antibody

For research use only

Citations
Reviews

Human/Mouse/Rat PP1 Inhibitor-2 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4719

Key Product Details

Species Reactivity

Validated:

Cited:

Applications