Western blot shows lysates of A549 human lung carcinoma cell line, WI-38 human lung fibroblast cell line, HepG2 human hepatocellular carcinoma cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat PINCH1 Monoclonal Antibody (Catalog # MAB6257) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for PINCH1 at approximately 37-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human/Mouse/Rat PINCH1 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: A549 human lung carcinoma cell line, WI‑38 human lung fibroblast cell line, HepG2 human hepatocellular carcinoma cell line, and NRK rat normal kidney cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PINCH1

Particularly interesting new Cys-His protein 1 (PINCH1), also known as LIMS1 (LIM and senescent cell antigen-like-containing domain protein 1), is a 37‑40 kDa, widely expressed LIM-only protein. Human PINCH1 is 325 amino acids (aa) in length and contains a tandem array of five LIM domains. Within the region used as an immunogen, human PINCH1 shares 100% and 99% aa sequence identity with mouse and rat PINCH1, respectively. PINCH1 is an effector of integrin and growth factor signaling, coupling surface receptors to downstream signaling molecules involved in the regulation of cell survival, cell proliferation and cell differentiation.

Long Name

Particularly Interesting New Cys-His Protein 1

Alternate Names

LIMS1

Entrez Gene IDs

3987 (Human)

Gene Symbol

LIMS1

UniProt

P48059 (Human)

Additional PINCH1 Products

Product Documents for Human/Mouse/Rat PINCH1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse/Rat PINCH1 Antibody

For research use only

Citations
Reviews

Human/Mouse/Rat PINCH1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6257

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all PINCH1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse/Rat PINCH1 Antibody

Detection of Human and Rat PINCH1 by Western Blot.