Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA or PMA and Ionomycin for 20 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-MEK1 (S218/S222)/ MEK2 (S222/S226) Monoclonal Antibody (Catalog # MAB8407) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-MEK1 (S218/S222)/ MEK2 (S222/S226) at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat Phospho-MEK1 (S218/S222)/MEK2 (S222/S226) Antibody

Application

Recommended Usage

Western Blot

0.5 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and HeLa human cervical epithelial carcinoma cell line treated with PMA or PMA and Ionomycin

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MEK1/MEK2

MEK1 and MEK2, also known as MAP2K1 and MAP2K2, are activated in response to mitogenic stimuli. Activation occurs through dual phosphorylation at S218/S222 of MEK1 and S222/S226 of MEK2 by several upstream MAP3Ks, including the Raf kinases. Both MEK1 and MEK2 are dual-specificity protein kinases, phosphorylating and activating ERK1 and ERK2 at Thr and Tyr positions.

Long Name

Mitogen-activated Protein Kinase Kinase 1

Alternate Names

CFC3, CFC4, dual specificity mitogen-activated protein kinase kinase 1, dual specificity mitogen-activated protein kinase kinase 2, ERK activator kinase 1, ERK activator kinase 2, MAP kinase kinase 2, MAPK/ERK kinase 1, MAPK/ERK kinase 2, MAPKK 1, MAPKK1, MAPKK2, MEK 1, MEK1, MEK2, MEL, mitogen-activated protein kinase kinase 2, p45, MKK1, MKK2, PRKMK1, PRKMK2, protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)

Additional MEK1/MEK2 Products

Product Documents for Human/Mouse/Rat Phospho-MEK1 (S218/S222)/MEK2 (S222/S226) Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human/Mouse/Rat Phospho-MEK1 (S218/S222)/MEK2 (S222/S226) Antibody

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.

For research use only

Citations
Reviews

Human/Mouse/Rat Phospho-MEK1 (S218/S222)/MEK2 (S222/S226) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8407

Key Product Details

Validated by

Species Reactivity

Applications

Label

Antibody Source

View all MEK1/MEK2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse/Rat Phospho-MEK1 (S218/S222)/MEK2 (S222/S226) Antibody

Detection of Human Phospho-MEK1 (S218/S222)/ MEK2 (S222/S226) by Western Blot.