Human/Mouse/Rat Phospho-HSP27 (S78/S82) Antibody

Name: Human/Mouse/Rat Phospho-HSP27 (S78/S82) Antibody
Brand: R&D Systems
SKU: AF2314

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF2314

Citations (2)

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Key Product Details

Species Reactivity:	Human, Mouse, Rat
Immunogen:	Phosphopeptide containing human HSP27 S78/S82 sites
Applications:	Immunocytochemistry/Immunofluorescence, Western Blot
Label:	Unconjugated
Source:	Polyclonal Rabbit IgG
Specificity:	Detects human HSP27 when dually phosphorylated at S78/S82, and mouse and rat HSP27 phosphorylated at S86.

View Full Technical Details for Human/Mouse/Rat Phospho-HSP27 (S78/S82) Antibody

Applications for Human/Mouse/Rat Phospho-HSP27 (S78/S82) Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	See below
Immunocytochemistry	5-15 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human/Mouse/Rat Phospho-HSP27 (S78/S82) Antibody

	Western Blot Detection of Human and Mouse Phospho-HSP27 (S78/S82) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line untreated (-) or treated (+) with 100 J/m²UV-C for 30 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-HSP27 (S78/S82) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2314), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-HSP27 (S78/S82) at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Immunocytochemistry Phospho-HSP27 (S78/S82) in HeLa Human Cell Line. HSP27 phosphorylated at S78/S82 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line unstimulated (lower panel) or stimulated with 20 mJ/cm²ultraviolet radiation (upper panel) using Rabbit Anti-Human/Mouse/Rat Phospho-HSP27 (S78/S82) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2314) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Formulation, Preparation and Storage

Purification	Antigen and protein A Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: HSP27

Heat shock proteins (HSPs) are a family of highly conserved stress response proteins. Heat shock proteins function primarily as molecular chaperones by facilitating the folding of other cellular proteins, preventing protein aggregation or targeting improperly folded proteins to specific degradative pathways. HSPs are typically expressed at low levels under normal physiological conditions but are dramatically up-regulated in response to cellular stress. Elevated levels of HSPs have been observed in association with ischemia/reperfusion, cancer, and chronic heart failure. HSP27 is a member of the small heat shock protein family, which also includes HSP25 and the alpha -crystallins. HSP27 forms a large oligomer and the extent of phosphorylation plays a role in determining specific functions. HSP27 also functions as an anti-apoptotic molecule, regulating apoptosis through direct interaction with key components of the apoptotic pathway. HSP27 binds and sequesters cytochrome c released from the mitochondria in response to an apoptotic stimulus. This prevents the proper assembly of the apoptosome and subsequently, the activation of procaspase-9 and procaspase-3.

Item	Value
References	Gusev, N.B. et al. (2002) Biochemistry (Moscow) 67:511. Garrido, C. et al. (2001) Biochem. Biophys. Res. Commun. 286:433. Garrido, C. (2002) Cell Death Diffr. 9:483. Brvey, J-M. et al. (2000) Nat. Cell Biol. 2:645.
Long Name	Heat Shock Protein 27
Entrez Gene IDs	3315 (Human); 15510 (Mouse); 24471 (Rat)
Alternate Names	28 kDa heat shock protein, DKFZp586P1322, Estrogen-regulated 24 kDa protein, HMN2B, HS.76067, HSP25, HSP27, HSP27HSP 27, HSP28CMT2F, HSPB1, Heat shock 27 kDa protein, SRP27, Stress-responsive protein 27, heat shock 27kD protein 1, heat shock 27kDa protein 1, heat shock protein beta-1

Citations for Human/Mouse/Rat Phospho-HSP27 (S78/S82) Antibody (2)

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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse/Rat Phospho-HSP27 (S78/S82) Antibody include:

Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

J Lee et al. (2018), A Novel Kinase Function of a Nucleoside-diphosphate-kinase Homolog in P. gingivalis is Critical in Subversion of Host Cell Apoptosis by Targeting Heat-Shock Protein 27 Cell. Microbiol., 0(0).
PMID: 29359393
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Lin et al. (2012), Survival of cancer stem cells under hypoxia and serum depletion via decrease in PP2A activity and activation of p38-MAPKAPK2-Hsp27. PLoS ONE, 7(11):e49605.
PMID: 23185379

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Product Documents for Human/Mouse/Rat Phospho-HSP27 (S78/S82) Antibody

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Human/Mouse/Rat Phospho-HSP27 (S78/S82) Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human/Mouse/Rat Phospho-HSP27 (S78/S82) Antibody

Scientific Data Examples for Human/Mouse/Rat Phospho-HSP27 (S78/S82) Antibody

Western Blot

Immunocytochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: HSP27

Citations for Human/Mouse/Rat Phospho-HSP27 (S78/S82) Antibody (2)

Product Documents for Human/Mouse/Rat Phospho-HSP27 (S78/S82) Antibody

Product Datasheets

COA

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