Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 µM pervanadate (PV) for 10 minutes and PC-12 rat adrenal pheochromocytoma cell line untreated or treated with 100 ng/mL Recombinant Rat beta-NGF (Catalog # 556-NG) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-FRS2 (Y436) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5126), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Bands were detected for Phospho-FRS2 (Y436) at approximately 70 - 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Phospho-FRS2 (Y436) in A431 Human Cell Line.

FRS2 phosphorylated at Y436 was detected in immersion fixed A431 human epithelial carcinoma cell line untreated (lower panel) or treated (upper panel) with pervanadate using Rabbit Anti-Human/Mouse/Rat Phospho-FRS2 (Y436) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5126) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Phospho-FRS2 (Y436) by Western Blot

INCB054828 inhibits FGFR-dependent signaling pathways.(A) KG1a or (B) RT-4 cells were treated with INCB054828 for 2 hours,lysed and subjected to immunoblotting for phospho- and total proteins in the FGFR signal transduction pathway including FGFR, ERK, FRS2, andSTAT5. (C) Concentration-dependent inhibition of phospho-FGFR3 byINCB054828 in RT-112 cells was determined using a proximity ligation assay with a mouse monoclonal anti-phospho-FGFR (Y653/Y654) and rabbitanti-FGFR. Original Western blot images are shown in S1 File (S1Raw images). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32315352), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse/Rat Phospho-FRS2 (Y436) Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed A431 human epithelial carcinoma cell line treated with pervanadate

Western Blot

1 µg/mL
Sample: Pervanadate-treated MCF-7 human breast cancer cell line and PC-12 rat adrenal pheochromocytoma cell line treated with Recombinant Rat beta-NGF (Catalog # 556-NG)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FRS2

FRS2 (FGF R substrate 2; also known as SNT and FRS2-alpha) is a 70‑90 kDa member of the FRS family of lipid-anchored docking proteins. It is an intermediary between FGF and RTK receptors and their Ras/MAPK signaling cascades. FRS2 contains a membrane-anchoring myristoylation signal (aa 1‑6), a PTB domain that interacts with FGF and NGF receptors (aa 13‑115), and a C-terminal tyrosine-rich region that serves as a docking site for GRB-2 and SHP-2 (aa 196‑471). Phosphorylation of Y436 by activated RTKs is required for efficient SHP-2 recruitment.

Long Name

Fibroblast Growth Factor Receptor Substrate 2

Alternate Names

FRS2alpha, SNT1

Entrez Gene IDs

10818 (Human); 327826 (Mouse)

Gene Symbol

FRS2

Additional FRS2 Products

Product Documents for Human/Mouse/Rat Phospho-FRS2 (Y436) Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse/Rat Phospho-FRS2 (Y436) Antibody

For research use only

Citations
Reviews

Human/Mouse/Rat Phospho-FRS2 (Y436) Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5126

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all FRS2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse/Rat Phospho-FRS2 (Y436) Antibody

Detection of Human and Rat Phospho-FRS2 (Y436) by Western Blot.