Human/Mouse/Rat PCK1 Alexa Fluor™ Plus 405-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7316AFP405
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Alexa Fluor Plus 405 (Excitation = 404 nm, Emission = 455 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PCK1
Specificity
Detects human, mouse, rat PCK1 in Western blots, and detects recombinant human PCK1 in direct ELISAs. In direct ELISAs, less than 3% cross-reactivity with recombinant mouse PCK1 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: PCK1
PCK1 (Phosphoenolpyruvate carboxykinase, cytosolic; also PEPCK-C) is a 67-69 kDa cytosolic member of the phosphoenolpyruvate kinase (GTP) family of enzymes. It is found in hepatocytes, adipocytes, intestinal and renal proximal tubule epithelial cells. PCK1 is one of two enzymes that drive the conversion of oxaloacetate plus GTP into PEP, GDP and CO2. The PEP generated in this reaction forms glucose via fructose phosphate intermediates. The other enzyme that has this activity is termed PEPCK-M and found in mitochondria. Human PCK1 is 622 amino acids (aa) in length. It contains a large catalytic region (aa 27-615) and three utilized acetylation sites. There is one potential alternative start site at Met315. Over aa 1-88, human PCK1 shares 80% aa sequence identity with mouse PCK1.
Long Name
Phosphoenolpyruvate Carboxykinase 1
Alternate Names
PEPCK-C, PEPCK1
Gene Symbol
PCK1
UniProt
Additional PCK1 Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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