Human/Mouse/Rat PARL Alexa Fluor® 594-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7306T
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Alexa Fluor 594 (Excitation = 590 nm, Emission = 617 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PARL
Gly71-Arg167
Accession # Q9H300
Gly71-Arg167
Accession # Q9H300
Specificity
Detects human, mouse, and rat PARL in Western blots and detects recombinant human PARL in direct ELISAs.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human/Mouse/Rat PARL Alexa Fluor® 594-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: PARL
PARL (Presenilins-associated Rhomboid-like protein; also P beta) is a 41-43 kDa member of the peptidase S54 family (alternatively, the 1 + 6 PARL subfamily; Rhomboid family) of molecules. It is ubiquitously expressed, being found in both embryo and adult. Rhomboid proteins are an ancient family of intramembrane-cleaving molecules that evolved as regulators of mitochondrial membrane dynamics. PARL is an inner mitochondrial-membrane embedded protein that appears to have at least two functions. First, it interacts with Opa1 and regulates apoptosis. Second, it appears to communicate to the nucleus information related to levels of mitochondrial stress. Human PARL is 379 amino acids (aa) in length. It consists of a mitochondrial targeting sequence (aa 1-52) plus a mature polypeptide (aa 53-379) that contains seven transmembrane segments and a 25 aa C-terminus that projects into the intermitochondrial membrane space. PARL undergoes two cleavages. The first occurs between Gly52Phe53 and removes the targeting sequence, generating a membrane-bound 37 kDa mature protein. The second occurs between Ser77Ala78, generating a membrane-bound 34 kDa processed form, plus a matrix-soluble 25 aa peptide (P beta-peptide; aa 53-77) that translocates to the nucleus. There is one potential splice variant that poseesses a Ser substitution for aa 203-253. Over aa 71-167, human PARL shares 92% aa sequence identity with mouse PARL.
Long Name
Presenilins Associated Rhomboid-like Protein
Alternate Names
PSARL1, RHBDS1
Gene Symbol
PARL
UniProt
Additional PARL Products
Product Specific Notices for Human/Mouse/Rat PARL Alexa Fluor® 594-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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