Human/Mouse/Rat NM23-H1/H2 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF6019

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AF6019
AF6019-SP
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all NM23-H1/H2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NM23‑H1
Met1-Glu152
Accession # P15531

Specificity

Detects human, mouse, and rat NM23‑H1/H2 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat NM23-H1/H2 Antibody

Detection of Human, Mouse, and Rat NM23-H1/H2 antibody by Western Blot.

Detection of Human, Mouse, and Rat NM23-H1/H2 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, HT-2 mouse T cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF Membrane was probed with 0.2 µg/mL of Human/Mouse/Rat NM23-H1/H2 Polyclonal Antibody (Catalog # AF6019) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for NM23-H1/H2 at approximately 17-20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
NM23-H1/H2 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC).

NM23‑H1/H2 in MCF‑7 Human Cell Line.

NM23-H1/H2 was detected in immersion fixed MCF-7 human breast cancer cell line using Sheep Anti-Human/Mouse/Rat NM23-H1/H2 Polyclonal Antibody (Catalog # AF6019) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Mouse/Rat NM23-H1/H2 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed MCF‑7 human breast cancer cell line

Western Blot

0.2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, HT‑2 mouse T cell line, and Rat‑2 rat embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NM23-H1/H2

NM23-H1 (Non-metastatic protein 23 homolog 1; also NDKA) is a 17‑20 kDa member of the NDK family of enzymes. NM23‑H1 is ubiquitous in expression, and performs multiple functions. It forms disulfide-linked homohexamers, and heterohexamers with NM23‑H2, generating a nucleoside diphosphate kinase that catalyzes a phosphoryl transfer from ATP to a nucleoside diphosphate. It also shows histidine protein and Ser/Thr protein kinase activity, and forms covalent linkages with molecules diverse as p53 and STRAP. It is found both intracellularly, and in blood at ng/mL concentrations. Human NM23-H1 is 152 amino acids (aa) in length, contains one NDP kinase domain (aa 5‑134) and shows acetylation at Ala2 and Lys56, plus phosphorylation at Tyr52, Thr94, Ser122 and Ser125. Human NM23-H1 shares 89% aa identity with human 17‑18 kDa NM23-H2  (Non-metastatic protein 23 homolog 2; also NDKB), and 94% aa identity with mouse NM23-H1. A second H1 isoform named NM23-H1B with 25 additional aa at the N-terminus has also been described.

Long Name

Non-metastatic Protein 23 Homolog 1/Homolog 2

Alternate Names

NM23H2

Entrez Gene IDs

4830 (Human)

Gene Symbol

NME1

UniProt

P15531

Additional NM23-H1/H2 Products

Product Documents for Human/Mouse/Rat NM23-H1/H2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human/Mouse/Rat NM23-H1/H2 Antibody

For research use only

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