Human/Mouse/Rat N-Cadherin Antibody

Name: Human/Mouse/Rat N-Cadherin Antibody
Brand: R&D Systems
SKU: AF6426
Price: 152 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF6426

Citations (11)

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Species Reactivity:	Human, Mouse, Rat
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human N‑Cadherin Asp160-Ala724 Accession # P19022
Applications:	CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Western Blot
Label:	Unconjugated
Source:	Polyclonal Sheep IgG
Specificity:	Detects human, mouse, and rat N‑Cadherin in Western blots. In direct ELISA, less than 10% cross-reactivity with recombinant human (rh) E-Cadherin, and rhR-Cadherin is observed.

View Full Technical Details for Human/Mouse/Rat N-Cadherin Antibody

Applications for Human/Mouse/Rat N-Cadherin Antibody

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
ELISA	This antibody functions as an ELISA detection antibody to detect human N-Cadherin when paired with Mouse Anti-Human N‑Cadherin Monoclonal Antibody (Catalog # MAB13883). This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human N-Cadherin DuoSet ELISA Kit (Catalog # ) for convenient development of a sandwich ELISA.
Flow Cytometry	2.5 µg/10⁶ cells	See below
Immunohistochemistry	1-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	5-15 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human/Mouse/Rat N-Cadherin Antibody

	Western Blot Detection of Human, Mouse, and Rat N‑Cadherin by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and rat brain tissue. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat N-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6426) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for N-Cadherin at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Flow Cytometry Detection of N‑Cadherin in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Sheep Anti-Human/Mouse/Rat N-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6426, filled histogram) or Sheep IgG control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).
	Immunocytochemistry N‑Cadherin in A549 Human Cell Line. N-Cadherin was detected in immersion fixed A549 human lung carcinoma cell line using Sheep Anti-Human/Mouse/Rat N-Cadherin Affinity-purified Polyclonal Antibody (Catalog # AF6426) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
	Immunohistochemistry N‑Cadherin in Human Heart N-Cadherin was detected in immersion fixed paraffin-embedded sections of human heart using Sheep Anti-Human/Mouse/Rat N-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6426) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Sheep IgG VisUCyte HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to intercalated disks between the myocardial cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
	ELISA Human N‑Cadherin ELISA Standard Curve. Recombinant Human N-Cadherin protein was serially diluted 2-fold and captured by Mouse Anti-Human N-Cadherin Monoclonal Antibody (Catalog # MAB13883) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human/Mouse/Rat N-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6426) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Sterile PBS to a final concentration of 0.2 mg/mL. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: N-Cadherin

N-Cadherin (Neural Cadherin; also CD325 and Cadherin-2) is a 130-135 kDa member of the "classical" (or type I) cadherin subfamily, cadherin superfamily of proteins. It is expressed on multiple cell types, including neurons, fibroblasts, Schwann cells, endothelial cells and hepatic stellate cells. N-Cadherin mediates homotypic binding, either in cis (same cell) or trans (adjacent cell). proN-Cadherin is expressed as an 881 amino acid (aa) type I transmembrane glycoprotein. It may be initially inserted into the ER, where the 15-20 kDa prodomain (aa 26-159) is cleaved by proprotein convertase, and the mature molecule (aa 160-906) is transported to the surface. Mature N-Cadherin contains a 565 aa extracellular region (aa 160-724) that possesses five cadherin domains (aa 160-714), and a 161 aa cytoplasmic tail that undergoes phosphorylation at Tyr785. There is one splice variant that contains a 10 aa substitution for aa 839-906. Over aa 160-724, human N Cadherin shares 98% aa identity with mouse N-Cadherin.

Item	Value
Long Name	Neural Cadherin
Entrez Gene IDs	1000 (Human); 12558 (Mouse); 83501 (Rat)
Alternate Names	ACOGS, ARVD14, CD325, CD325 antigen, CDH2, CDHN, CDw325, Cadherin-2, N-Cadherin, N-cadherin 1, NCAD, NCadherin, Neural cadherin, cadherin 2, type 1, N-cadherin (neuronal), calcium-dependent adhesion protein, neuronal, neural-cadherin

Citations for Human/Mouse/Rat N-Cadherin Antibody (11)

Showing 1 - 10 of 11 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse/Rat N-Cadherin Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: ICC

RR Khadim et al. (2022-01-01), Integrating Oxygen and 3D Cell Culture System: A Simple Tool to Elucidate the Cell Fate Decision of hiPSCs International Journal of Molecular Sciences, 23(13).
PMID: 35806277
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

K Karnas et al. (2021-01-01), Specific Binding of Novel SPION-Based System Bearing Anti-N-Cadherin Antibodies to Prostate Tumor Cells International Journal of Nanomedicine, 16(0):6537-6552.
PMID: 34602817
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot

D Basak et al. (2021-01-01), Reciprocal interplay between asporin and decorin: Implications in gastric cancer prognosis PLoS ONE, 16(8):e0255915.
PMID: 34379688
Species: Mouse
Sample Types: Protein
Applications: Western Blot

JH Wu et al. (2020-01-01), Inhibition of Sema4D/PlexinB1 signaling alleviates vascular dysfunction in diabetic retinopathy EMBO Mol Med, 12(2):e10154.
PMID: 31943789
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

JL Harder et al. (2019-01-01), Organoid single cell profiling identifies a transcriptional signature of glomerular disease JCI Insight, 4(1).
PMID: 30626756
Species: Mouse
Sample Types: Protein
Applications: Western Blot

A Ikeda et al. (2019-01-01), ADAM10 is Expressed by Ameloblasts, Cleaves the RELT TNF Receptor Extracellular Domain and Facilitates Enamel Development Sci Rep, 9(1):14086.
PMID: 31575895
Species: Human
Sample Types: Whole Cells
Applications: ICC

Y Su et al. (2019-01-01), Phenotypic heterogeneity and evolution of melanoma cells associated with targeted therapy resistance PLoS Comput. Biol., 15(6):e1007034.
PMID: 31166947
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot

Y Wan et al. (2018-01-01), MicroRNA-149-5p regulates blood-brain barrier permeability after transient middle cerebral artery occlusion in rats by targeting S1PR2 of pericytes FASEB J., 0(0):fj201701121R.
PMID: 29401609
Species: Human
Sample Types: Whole Cells
Applications: ICC

E Cherubini et al. (2017-01-01), BDNF/TrkB axis activation promotes epithelial-mesenchymal transition in idiopathic pulmonary fibrosis J Transl Med, 15(1):196.
PMID: 28938915
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Guan X et al. (2013-01-01), MMP20 modulates cadherin expression in ameloblasts as enamel develops. , 92(0).
PMID: 24067343
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Windus L et al. (2012-01-01), In vivo biomarker expression patterns are preserved in 3D cultures of Prostate Cancer. Exp Cell Res, 318(19):2507-19.
PMID: 22841689

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Product Documents for Human/Mouse/Rat N-Cadherin Antibody

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Human/Mouse/Rat N-Cadherin Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Applications for Human/Mouse/Rat N-Cadherin Antibody

Scientific Data Examples for Human/Mouse/Rat N-Cadherin Antibody

Western Blot

Flow Cytometry

Immunocytochemistry

Immunohistochemistry

ELISA

Formulation, Preparation and Storage

Reconstitution Calculator

Background: N-Cadherin

Citations for Human/Mouse/Rat N-Cadherin Antibody (11)

Product Documents for Human/Mouse/Rat N-Cadherin Antibody

Product Datasheets

COA

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