Human/Mouse/Rat Mitofusin 2 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF7884

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AF7884
AF7884-SP
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Mitofusin 2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Mitofusin 2
Arg364-Phe599
Accession # O95140

Specificity

Detects human, mouse, and rat Mitofusin 2 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human Mitofusin 1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Mitofusin 2 Antibody

Mitofusin 2 antibody in Mouse Skeletal Muscle by Immunohistochemistry (IHC-Fr).

Mitofusin 2 in Mouse Skeletal Muscle.

Mitofusin 2 was detected in perfusion fixed frozen sections of mouse skeletal muscle using Sheep Anti-Human/Mouse/Rat Mitofusin 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7884) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Human/Mouse/Rat Mitofusin 2 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse skeletal muscle

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mitofusin 2

MFN-2 (Mitofusin 2; also Hypertension-related protein 1, Mitochondrial assembly regulatory factor, HSG protein, and Transmembrane GTPase MFN-2) is an 85‑100 kDa mitochondrial member of the dynamin family of molecules. It is ubiquitously expressed, and found in both the ER and outer mitochondrial membrane. Through trans-interactions with MFN-1 and/or MFN-2, MFN-2 apparently mediates the fusion of individual mitochondria with either the ER or with adjacent mitochondria. Pertubations with this process result in both a general failure of mitochondria to fuse, and in cell-specific effects such as a reduction in oxidative phosphorylation, a decrease in the axonal transport of mitochondria, and a deficit in the expression of respiratory chain components. MFN-2 has two key domains. One is a coiled-coil region that mediates MFN-2:MFN-1/2 binding, and a second is a GTPase domain that likely plays a role in fusion. While active, the MFN-2 GTPase domain is 8-fold lower in activity than that for MFN-1. Human MFN-2 is a 757 amino acid (aa) two transmembrane protein that contains a cytoplasmic N- and C‑terminus. The cytoplasmic N‑terminus (aa 1-604) contains the GTPase domain (aa 99-258) while the cytoplasmic C‑terminus possesses a critical coiled-coil motif (aa 696-738). MFN-2 is known to form oligomers, either with itself or MFN-1. Two potential splice variants are reported, one that shows a deletion of aa 245-273, and a second that contains a 33 aa substitution for aa 573-757, suggesting this might be a soluble for of MFN-2. Over aa 364-599, human and mouse MFN-2 share 93% aa sequence identity. Full-length human MFN-2 and MFN-1 share 61% aa sequence identity.

Alternate Names

CMT2A, CMT2A2, CPRP1, HSG, MARF, MFN2

Entrez Gene IDs

9927 (Human)

Gene Symbol

MFN2

UniProt

O95140

Additional Mitofusin 2 Products

Product Documents for Human/Mouse/Rat Mitofusin 2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human/Mouse/Rat Mitofusin 2 Antibody

For research use only

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