Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Polyclonal Sheep IgG

View all Mitofusin 2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Mitofusin 2
Arg364-Phe599
Accession # O95140

Specificity

Detects human, mouse, and rat Mitofusin 2 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human Mitofusin 1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Mitofusin 2

MFN-2 (Mitofusin 2; also Hypertension-related protein 1, Mitochondrial assembly regulatory factor, HSG protein, and Transmembrane GTPase MFN-2) is an 85‑100 kDa mitochondrial member of the dynamin family of molecules. It is ubiquitously expressed, and found in both the ER and outer mitochondrial membrane. Through trans-interactions with MFN-1 and/or MFN-2, MFN-2 apparently mediates the fusion of individual mitochondria with either the ER or with adjacent mitochondria. Pertubations with this process result in both a general failure of mitochondria to fuse, and in cell-specific effects such as a reduction in oxidative phosphorylation, a decrease in the axonal transport of mitochondria, and a deficit in the expression of respiratory chain components. MFN-2 has two key domains. One is a coiled-coil region that mediates MFN-2:MFN-1/2 binding, and a second is a GTPase domain that likely plays a role in fusion. While active, the MFN-2 GTPase domain is 8-fold lower in activity than that for MFN-1. Human MFN-2 is a 757 amino acid (aa) two transmembrane protein that contains a cytoplasmic N- and C‑terminus. The cytoplasmic N‑terminus (aa 1-604) contains the GTPase domain (aa 99-258) while the cytoplasmic C‑terminus possesses a critical coiled-coil motif (aa 696-738). MFN-2 is known to form oligomers, either with itself or MFN-1. Two potential splice variants are reported, one that shows a deletion of aa 245-273, and a second that contains a 33 aa substitution for aa 573-757, suggesting this might be a soluble for of MFN-2. Over aa 364-599, human and mouse MFN-2 share 93% aa sequence identity. Full-length human MFN-2 and MFN-1 share 61% aa sequence identity.

Alternate Names

CMT2A, CMT2A2, CPRP1, HSG, MARF, MFN2

Entrez Gene IDs

9927 (Human)

Gene Symbol

MFN2

UniProt

O95140

Additional Mitofusin 2 Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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