Human/Mouse/Rat MDM2/HDM2 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF1244
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
E. coli-derived recombinant human MDM2
Specificity
Detects MDM2 in Western blots.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Applications for Human/Mouse/Rat MDM2/HDM2 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human MDM2
Recombinant Mouse MDM2
Recombinant Rat MDM2
Sample: Recombinant Human MDM2
Recombinant Mouse MDM2
Recombinant Rat MDM2
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MDM2/HDM2
MDM2 is a key regulator of p53 tumor suppressor protein activity and stability. MDM2 binds to and inhibits the transactivation domain of p53. In addition, MDM2 controls p53 stability by functioning as its E3 ligase in ubiquitination and by shuttling p53 from the nucleus to the cytoplasm for subsequent degradation. The importance of the p53/MDM2 relationship is underscored by the existence of an autoregulatory feedback loop whereby activated p53 transcriptionally upregulates the expression of its own inhibitor, MDM2.
Long Name
Double Minute 2 Protein
Alternate Names
HDM2
Gene Symbol
MDM2
Additional MDM2/HDM2 Products
Product Documents for Human/Mouse/Rat MDM2/HDM2 Biotinylated Antibody
Product Specific Notices for Human/Mouse/Rat MDM2/HDM2 Biotinylated Antibody
For research use only
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