Detects human, mouse, and rat LAR in Western blots. In Western blots, this antibody detects both full-length protein and the cleaved LAR extracellular (E) domain. The antibody does not cross react with PTPR sigma.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat LAR Antibody

Detection of Human/Mouse/Rat LAR by Western Blot.

Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Neuro-2A mouse neuroblastoma cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat LAR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3004) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Bands were detected for LAR at approximately 207 kDa for full-length LAR and 139 kDa for cleaved LAR (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat LAR Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, Neuro-2A mouse neuroblastoma cell line, and Y3-Ag rat myeloid cell line

Reviewed Applications

Read 1 review rated 5 using AF3004 in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAR

Leucocyte Antigen-Related (LAR) tyrosine phosphatase, also known as Protein Tyrosine Phoshatase, Receptor-type F (PTPRF), is an integral membrane protein with a non-glycosylated molecular weight of 207 kDa. The extracellular domain is cleaved near the cell membrane by a subtilisin-like endoprotease to a molecular weight of 139 kDa. Depending on cellular conditions, the extracellular domain may remain associated with the rest of the molecule or can be shed into the extracellular medium. This antibody was made against the extracellular domain of LAR and detects both full-length and cleaved LAR.

Long Name

Leukocyte Antigen-Related Tyrosine Phosphatase

Alternate Names

PTPRF

Entrez Gene IDs

5792 (Human); 104121 (Mouse)

Gene Symbol

PTPRF

UniProt

NP_569707

Additional LAR Products

Product Documents for Human/Mouse/Rat LAR Antibody

Product Datasheets

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COA

SDS

Download SDS

Product Specific Notices for Human/Mouse/Rat LAR Antibody

For research use only

Citations
Reviews

Human/Mouse/Rat LAR Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3004

Key Product Details

Species Reactivity

Validated:

Cited:

Applications