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Human/Mouse/Rat JNK1/JNK2 Antibody
R&D Systems, Inc. a Bio-Techne Brand
Catalog # MAB2076
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Key Product Details
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | E. coli-derived recombinant human JNK1 isoform 2 |
| Applications: | Immunocytochemistry/Immunofluorescence, Simple Western, Western Blot |
| Label: | Unconjugated |
| Source: | Monoclonal Mouse IgG2A Clone # 252323 |
| Specificity: |
Detects human, mouse, and rat p46 and p54 JNK1/JNK2. Does not detect recombinant JNK3. |
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Western BlotDetection of Human/Mouse/Rat JNK1/JNK2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.2 µg/mL Mouse Anti-Human/Mouse/Rat JNK1/JNK2 Monoclonal Antibody (Catalog # MAB2076) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human JNK1, JNK2, and JNK3 (1 ng/lane) were included. Specific bands for JNK1 and JNK2 were detected at approximately 46 kDa and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
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ImmunocytochemistryJNK1/JNK2 in HeLa Human Cervical Epithelial Carcinoma Cell Line. JNK1/JNK2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat JNK1/JNK2 Monoclonal Antibody (Catalog # MAB2076) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
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ImmunocytochemistryJNK1/JNK2 in C2C12 Mouse Cell Line. JNK1/JNK2 was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Human/Mouse/Rat JNK1/JNK2 Monoclonal Antibody (Catalog # MAB2076) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
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Simple WesternDetection of Human JNK1/JNK2 by Simple WesternTM. Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line, loaded at 0.2 mg/mL. Specific bands were detected for JNK1/JNK2 at approximately 48 and 58 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat JNK1/JNK2 Monoclonal Antibody (Catalog # MAB2076). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
Citations for Human/Mouse/Rat JNK1/JNK2 Antibody (4)
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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse/Rat JNK1/JNK2 Antibody include:
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Species: Human
Sample Types: Cell Lysate
Applications: Western BlotYY Liu et al. (2020), Activity of fibroblast-like synoviocytes in rheumatoid arthritis was impaired by dickkopf-1 targeting siRNA Chin. Med. J., 0(6):679-686.
PMID: 32068606 -
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western BlotM Fang et al. (2018), Ulinastatin Ameliorates Pulmonary Capillary Endothelial Permeability Induced by Sepsis Through Protection of Tight Junctions via Inhibition of TNF-? and Related Pathways Front Pharmacol, 9(0):823.
PMID: 30150933 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotS Janczar et al. (2017), WWOX sensitises ovarian cancer cells to paclitaxel via modulation of the ER stress response Cell Death Dis, 8(7):e2955.
PMID: 28749468 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotChavez-Galan L et al. (2013), Pre-exposure of Mycobacterium tuberculosis-infected macrophages to crystalline silica impairs control of bacterial growth by deregulating the balance between apoptosis and necrosis. PLoS ONE, 8(11):e80971.
PMID: 24278357
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