Human/Mouse/Rat JNK1 Antibody

Name: Human/Mouse/Rat JNK1 Antibody
Brand: R&D Systems
SKU: MAB17761
Rating: 5 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB17761

Citations (3)

(2)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Species Reactivity:	Human, Mouse, Rat
Immunogen:	E. coli-derived recombinant human JNK1 isoform 2 Ser2-Ile384 Accession # P45983-1
Applications:	Immunocytochemistry/Immunofluorescence, Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG₁ Clone # 228601
Specificity:	Detects human JNK1 in direct ELISAs and human, mouse, and rat JNK1 expressed predominantly as p46 JNK (isoforms 2 and/or 3, both 384 aa), with lesser amounts of p54 JNK (isoforms 1 and/or 4, both 427 aa), in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) JNK2 or rhJNK3 is observed.

View Full Technical Details for Human/Mouse/Rat JNK1 Antibody

Applications for Human/Mouse/Rat JNK1 Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Immunocytochemistry	8-25 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human/Mouse/Rat JNK1 Antibody

	Western Blot Detection of Human JNK1 by Western Blot. Western blot shows recombinant human JNK1, JNK2, and JNK3 (1 ng/lane). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB17761) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for JNK1 was detected at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
	Western Blot Detection of Human, Mouse, and Rat JNK1 by Western Blot. Western blot shows lysates of CHP-100 human neuroblastoma cell line, C6 rat glioma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB17761) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for JNK1 was detected at approximately 46 kDa and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
	Immunocytochemistry JNK1 in MCF‑7 Human Cell Line. JNK1 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB17761) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS containing 0.02% NaN₃.
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: JNK1

The c-Jun N-terminal kinases (JNKs) are encoded by three genes: JNK1, JNK2, and JNK3. Members of the MAPK superfamily, JNKs are activated by environmental stresses and inflammatory cytokines. JNK1, also known as SAPK1 gamma and MAPK8, is expressed as four isoforms generated by alternative splicing. JNK1 is activated by dual phosphorylation at T183 and Y185 by the MAPK kinases MKK4 and/or MKK7.

Item	Value
Long Name	C-Jun N-terminal Kinase 1
Entrez Gene IDs	5599 (Human); 26420 (Mouse); 116554 (Rat)
Alternate Names	EC 2.7.11, EC 2.7.11.24, JNK-46, JNK1, JNK1 alpha protein kinase, JNK1 beta protein kinase, JNK1JNK1A2, JUN N-terminal kinase, MAP kinase 8, MAPK 8, MAPK8, PRKM8, PRKM8JNK, SAPK1, SAPK1JNK21B1/2, Stress-activated protein kinase 1, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, mitogen-activated protein kinase 8, mitogen-activated protein kinase 8 isoform JNK1 alpha1, mitogen-activated protein kinase 8 isoform JNK1 beta2, protein kinase JNK1

Citations for Human/Mouse/Rat JNK1 Antibody (3)

Showing 1 - 3 of 3 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse/Rat JNK1 Antibody include:

Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

C Liu et al. (2017), APP upregulation contributes to retinal ganglion cell degeneration via JNK3 Cell Death Differ., 0(0).
PMID: 29238071
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

(2016), Phosphorylation Sites Identified in the NEIL1 DNA Glycosylase Are Potential Targets for the JNK1 Kinase PLoS ONE, 11(8):e0157860.
PMID: 27518429
Species: Human
Sample Types: Cell Lysates
Applications: ELISA Development

Kato T et al. (2008), Distinct role of c-Jun N-terminal kinase isoforms in human neutrophil apoptosis regulated by tumor necrosis factor-alpha and granulocyte-macrophage colony-stimulating factor. J. Interferon Cytokine Res., 28(4):235-43.
PMID: 18439101

There are no citations that match your criteria.

Showing 1 - 3 of 3 citations Showing All

Customer Reviews for Human/Mouse/Rat JNK1 Antibody (2)

4.5 out of 5

2 customer ratings

5 stars

50%

4 stars

50%

3 stars

2 stars

1 star

Customer Images

Showing 1 - 2 of 2 reviews Showing All

Filter By:

Human/Mouse/Rat JNK1 Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: Epithelial cells

Species: Mouse

Verified Customer | Posted 01/26/2022
Human/Mouse/Rat JNK1 Antibody

Name: Anonymous

Application: WB

Sample Tested: Prostate cancer

Species: Human

Verified Customer | Posted 01/25/2018

There are no reviews that match your criteria.

Showing 1 - 2 of 2 reviews Showing All

Product Documents for Human/Mouse/Rat JNK1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Human/Mouse/Rat JNK1 Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased

Applications for Human/Mouse/Rat JNK1 Antibody

Scientific Data Examples for Human/Mouse/Rat JNK1 Antibody

Western Blot

Western Blot

Immunocytochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: JNK1

Citations for Human/Mouse/Rat JNK1 Antibody (3)

Customer Reviews for Human/Mouse/Rat JNK1 Antibody (2)

Customer Images

Human/Mouse/Rat JNK1 Antibody

Human/Mouse/Rat JNK1 Antibody

Product Documents for Human/Mouse/Rat JNK1 Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected