Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 42° C heat shock for 30 minutes and allowed to recover for 3 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat HSP70/HSPA1A Monoclonal Antibody (Catalog # MAB16631) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HSP70/HSPA1A at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human. Mouse, and Rat HSP70/HSPA1A by Western Blot.

Western blot shows lysates of human liver tissue, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat HSP70/HSPA1A Monoclonal Antibody (Catalog # MAB16631) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HSP70/HSPA1A at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

HSP70/HSPA1A in Jurkat Human Cell Line.

HSP70/HSPA1A was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Mouse Anti-Human/Mouse/Rat HSP70/HSPA1A Monoclonal Antibody (Catalog # MAB16631) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Applications for Human/Mouse/Rat HSP70/HSPA1A Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed Jurkat human acute T cell leukemia cell line

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney cancer tissue

Simple Western

20 µg/mL
Sample: Jurkat human acute T cell leukemia cell line treated by heat shock

Western Blot

2 µg/mL
Sample: Jurkat human acute T cell leukemia cell line treated by heat shock, human liver tissue, mouse liver tissue, and rat liver tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HSP70/HSPA1A

Heat shock proteins (HSPs) are a family of highly conserved stress response proteins. Heat shock proteins function primarily as molecular chaperones by facilitating the folding of other cellular proteins, preventing protein aggregation or targeting improperly folded proteins to specific degradative pathways. HSPs are typically expressed at low levels under normal physiological conditions but are dramatically up‑regulated in response to cellular stress. HSP70 is a 72 kDa member of the heat shock protein 70 family of proteins. HSP70, also known as HSPA1A, HSP70-1, and HSP72 is a 641 amino acid (aa) heat shock protein. Over aa 1-641, human HSP70 shares 95% and 97% aa identity to mouse and rat HSP70.

Long Name

Heat Shock Protein 70

Alternate Names

HSP70-1A, HSP72, HSPA1A

Entrez Gene IDs

3303 (Human); 15511 (Mouse); 24472 (Rat)

Gene Symbol

HSPA1A

UniProt

P0DMV8 and P0DMN9

Additional HSP70/HSPA1A Products

Product Documents for Human/Mouse/Rat HSP70/HSPA1A Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human/Mouse/Rat HSP70/HSPA1A Antibody

For research use only

Citations
Reviews

Human/Mouse/Rat HSP70/HSPA1A Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB16631

Key Product Details

Validated by

Species Reactivity

Applications

Label

Antibody Source

View all HSP70/HSPA1A Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse/Rat HSP70/HSPA1A Antibody

Detection of Human HSP70/HSPA1A by Western Blot.