Western blot shows lysates of MCF-7 human breast cancer cell line, C2C12 mouse myoblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat FRS2 Monoclonal Antibody (Catalog # MAB4069) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FRS2 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat FRS2 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: MCF-7 human breast cancer cell line, C2C12 mouse myoblast cell line, and PC-12 rat adrenal pheochromocytoma cell line

Reviewed Applications

Read 1 review rated 4 using MAB4069 in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FRS2

FRS2 (FGF R substrate 2; also known as SNT and FRS2 alpha) is a 70‑90 kDa member of the FRS family of lipid-anchored docking proteins. It is an intermediary between FGF R and other RTK receptors and their Ras/MAPK signaling cascades. FRS2 contains a membrane-anchoring myristoylation signal (aa 1‑6), a PTB domain (aa 13‑115) that interacts with FGF and NGF receptors, and a C-terminal Tyr-rich region (aa 196‑471) that serves as a docking site for GRB‑2 and SHP‑2.

Long Name

Fibroblast Growth Factor Receptor Substrate 2

Alternate Names

FRS2alpha, SNT1

Entrez Gene IDs

10818 (Human); 327826 (Mouse)

Gene Symbol

FRS2

UniProt

Q8WU20

Additional FRS2 Products

Product Documents for Human/Mouse/Rat FRS2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse/Rat FRS2 Antibody

For research use only

Citations
Reviews

Human/Mouse/Rat FRS2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4069

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all FRS2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse/Rat FRS2 Antibody

Detection of Human/Mouse/Rat FRS2 by Western Blot.