Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all FKBP13 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FKBP13
Ala22-Leu142
Accession # P26885

Specificity

Detects endogenous human, mouse, and rat FKBP13 in Western blots. In Western blots, this antibody does not cross‑react with recombinant human FKBP12, FKBP25, FKBP38, FKBP51 or FKBP52.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat FKBP13 Antibody

Detection of Human/Mouse/Rat FKBP13 antibody by Western Blot.

Detection of Human/Mouse/Rat FKBP13 by Western Blot.

Western blot shows lysates of Rat-2 rat embryonic fibroblast cell line and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat FKBP13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4356) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FKBP13 at approximately 13 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat FKBP13 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Rat-2 rat embryonic fibroblast cell line and Neuro-2A mouse neuroblastoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FKBP13

FK506 binding protein, 13 kilodalton molecular weight (FKBP13), also called FKBP2, is a peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans‑proline residues critical for proper folding of proteins. The immunosuppressants FK506 and rapamycin are potent inhibitors of FKBP13. This isomerase is localized in the endoplasmic reticulum and is also associated with cytoskeletal elements such as erythrocyte band 4.1. FKBP13 levels are increased by treatments that affect golgi protein processing such as tunicamycin and heat shock, suggesting that FKBP13 is important in the unfolded protein response observed with endoplasmic reticulum stress.

Long Name

13 kDa FK506 Binding Protein

Alternate Names

FKBP2

Entrez Gene IDs

2286 (Human); 14227 (Mouse); 25639 (Rat)

Gene Symbol

FKBP2

UniProt

P26885

Additional FKBP13 Products

Product Documents for Human/Mouse/Rat FKBP13 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat FKBP13 Antibody

For research use only

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